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ID   PAX11      preliminary; circular DNA; SYN; 3130 BP.
AC   IG5084;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pAX11 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pK18, pK19 from pUC19
RC   plasmid from pK19
RC   pKpra from plasmid & pRIT11, protein A
RC   p1 from pKpra
RC   pAX11, pAX13 from p1 & oligo & pEX11
RC   pAX12 from p1 & oligo & pEX12
RA   Zueco J., Boyd A.;
RT   "Protein A fusion vectors for use in combination with pEX vectors in
RT   the production and affinity purification of specific antibodies.";
RL   Gene 121:181-182(1992).
CC   pEX11, pEX12, pEX13 MCS. not original sequence.
CC   NM (pAX11)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pK19)(pRIT11)
CC   BR (pAX12)(pAX13)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   -> pK19 2700bp
FT                   1. pK19 EcoRI 2700bp, pUC19 397..397
FT                   Klenow
FT                   -> plasmid 2700bp
FT                   1. pBR322 4361bp
FT                   2. Staphylococcus, spa gene
FT                   -> pSPA2
FT                   1. pBR322 EcoRV 4361bp 188..188
FT                   2. Staphylococcus EcoRV-EcoRV 2100bp, spa gene
FT                   -> pSPA5 9300bp
FT                   1. pBR322 remove ClaI-EcoRV 163bp 25..188, 4198bp
FT                   2. pSPA5 EcoRV-EcoRV 2100bp, Staphylococcus spa gene
FT                   TaqI-EcoRV 1770bp, Staphylococcus spa gene/promoter
FT                   -> pSPA8 5800bp
FT                   1. pUR222 2700bp
FT                   2. pSPA5 2000bp, Staphylococcus spa gene
FT                   -> plasmid 5000bp
FT                   1. plasmid 5000bp
FT                   2. pSPA2
FT                   -> pSPA10
FT                   1. pHV33 7271bp
FT                   2. pSPA10
FT                   3. pSPA8
FT                   -> pSPA16
FT                   1. pSPA16
FT                   2. pEMBL9+ 3939bp
FT                   -> pRIT3
FT                   1. pRIT3
FT                   2. pC194 2910bp
FT                   -> pRIT4
FT                   1. pRIT4
FT                   linker
FT                   -> pRIT11
FT                   1. plasmid remove BamHI-HindIII 30bp, pUC19 418..448
FT                   2. pRIT11 BamHI-HindIII, protein A gene/spa gene
FT                   -> pKpra
FT                   1. pKpra remove SmaI-SmaI, MCS
FT                   -> p1
FT                   1. p1 EcoRI
FT                   2. oligo 8bp aattggcc
FT                   -> plasmid2
FT                   1. plasmid2 AvaI-KpnI
FT                   2. oligo 17bp ccgggctgcagccgtac
FT                   -> plasmid3
FT                   1. plasmid3 remove small SmaI-PstI
FT                   2. pEX11 SmaI-PstI 29bp 3204..3233, MCS
FT                   -> pAX11 3130bp"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             0..0
FT                   /note="GEN E. coli spa gene"
FT   misc_binding    0..0
FT                   /note="MCS SmaI-KpnI-EcoRI-SpeI-SphI-BamHI-SalI-PstI"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
FT   CDS             0..0
FT                   /note="GEN E. coli kanamycin gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 57 BP; 11 A; 19 C; 16 G; 11 T; 0 other;
     gccaattccc ggggtaccga attcactagt gcatgcggat ccgtcgacct gcagccg