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ID   PAZE3SS    preliminary; circular DNA; SYN; 58 BP.
AC   M18287;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pAZe3ss - incomplete, MCS.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-58
RC   pRMe1 from pRMt121 & pPLc28 & pMC1403
RC   pAZe3 from pBGH006 & pMC1403
RC   pRMe2 from pBGH006 & pMC1403
RC   pRM0ss from pKTH605 & pPLc28
RC   pRMe1ss from pRMe1 & pRM0ss
RC   pRMe2ss from pRMe2 & pRM0ss
RC   pAZe3ss from pAZe3 & pRM0ss
RC   pHAe309, pHAe315, pHAe320, pHAe323, pHAe308 from HA gene & pAZe3
RA   Zaballos A., Salas M., Mellado R.P.;
RT   "A set of expression plasmids for the synthesis of fused and unfused
RT   polypeptides in Escherichia coli";
RL   Gene 58:67-76(1987).
CC   NCBI gi: 208359
CC   NM (pAZe3ss)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 PstI 4361bp 3612..3612, amp
FT                   terminal transferase dG tail
FT                   2. bGH gene
FT                   S1 nuclease
FT                   terminal transferase dC tail 763bp, bGH gene
FT                   -> pBGH001 5124bp
FT                   1. pBGH001 PstI-PstI 763bp, bGH gene
FT                   S1 nuclease
FT                   BamHI linker 8bp cggatccg:BamHI linker 8bp cggatccg
FT                   BamHI-BamHI 763bp, bGH gene
FT                   2. pBR322 BamHI 4361bp 376..376, tet
FT                   -> pBGH002 5100bp
FT                   1. pBGH002 NarI 5100bp 1207..1207
FT                   \ NarI = 415 436 550 1207
FT                   S1 nuclease
FT                   HindIII linker 10bp ccaagcttgg
FT                   HindIII-BamHI 800bp, bGH gene
FT                   2. pBR322 remove HindIII-BamHI 346bp 30..376,
FT                   \ tet/4015bp
FT                   -> pBGH003 4800bp
FT                   1. pBGH003 HindIII 4800bp, pBR322 30..30
FT                   S1 nuclease
FT                   -> pBGH004 4500bp [NcoI at beginning of bGH]
FT                   1. pBGH004 remove small NcoI-EcoRI, bGH to pBR322 4360
FT                   2. phage Mu HindIII-HaeIII 115bp, ner gene RBS/ATG
FT                   NcoI, at beginning of bGH
FT                   NcoI-EcoRI, ner gene RBS
FT                   -> pBGH005 4000bp
FT                   1. pPLa8 BamHI 3716bp, pBR322 3614..3614
FT                   2. linker BamHI-EcoRI-lac operator-EcoRI-BamHI
FT                   -> pPLa83 4000bp
FT                   1. pPLa83 remove EcoRI-EcoRI, lac operator/4000bp
FT                   -> pPLa831 4000bp
FT                   1. pPLa831 BamHI 4000bp, pBR322 3614..3614
FT                   2. linker BamHI-HindIII-HindIII-BamHI
FT                   -> pPLa832 4000bp
FT                   1. pPLa832 HindIII-BamHI 1200bp, oL/pL/kan gene frag
FT                   2. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> pPLc2 5200bp [2 EcoRI]
FT                   1. pPLc2 remove small HindIII-XhoI, 5000bp
FT                   BAL31 nuclease
FT                   -> pPLc23 5000bp [unique EcoRI, 3 HincII]
FT                   1. pPLc23 BamHI 5000bp, pBR322 3614..3614
FT                   2. linker BamHI-HindIII-HindIII-BamHI
FT                   -> pPLc236 5000bp [BalI, PvuII 2067, 2 HindIII]
FT                   1. pBGH005 EcoRI-BamHI, phage Mu/bGH gene
FT                   2. pPLc236 large EcoRI-BamHI, lambda pL promoter
FT                   -> pBGH006 5200bp1. pBR322 PstI 4361bp 3612..3612, amp
FT                   1. pBGH006 PstI-NcoI 1200bp, lambda pL/Mu ner RBS
FT                   :blunt end
FT                   2. pMC1403 SmaI-PstI 9065bp 3647..9820..2892, lacZ
FT                   -> pAZe3 10300bp
FT                   1. pKTH605 HindIII-BamHI 32bp 30..62,
FT                   \ 3-frame stop codons
FT                   2. pPLc28 remove small BamHI-HindIII 10bp, 2710bp
FT                   -> pRM0ss 2800bp
FT                   1. pAZe3 remove PstI-BamHI 758bp, pMC1403 2892..3650
FT                   \ 9500bp
FT                   2. pRM0ss BamHI-PstI 779bp, 3-frame stop codons
FT                   \ pKTH605 3300..4049..30
FT                   -> pAZe3ss 2900bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 58 BP; 15 A; 9 C; 17 G; 17 T; 0 other;
     aggagggttt ttaccatggg ggatccggtg attgattgat caatcaatca gcaagctt