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ID   PB101TP    preliminary; circular DNA; SYN; 57 BP.
AC   M18741;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Dictyostelium plasmid vector pB101TP1/pB101TP2 - incomplete, MCS.
KW   cloning vector; multiple cloning site; transformation vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-57
RC   pB10 from pEMBL18+ & B10 neo gene
RC   pB10T from pB10 & pDdG10
RC   pB101TP1 from pB10T & linker
RC   pB10R1 from pB10
RC   pB10RT from pB10R1 & pDdG10
RC   pB10TP2 from pB10RT & linker
RA   Early A.E., Williams J.G.;
RT   "Two vectors which facilitate gene manipulation and simplified
RT   transformation procedure for Dictyostelium discoideum";
RL   Gene 59:99-106(1987).
RN   [2]
RC   pDd2, pDd3, pDd7, pDd11.7.10 from Dictyostelium cysteine proteinase
RA   Williams J.G., North M.J., Mahbubani H.;
RT   "A developmentally regulated cysteine proteinase in Dictyostelium
RT   discoideum";
RL   EMBO J. 4:999-1006(1985).
RN   [3]
RC   B10, B12 from pBR322 & Tn5 & D.discoideum actin 6 gene
RC   B10.DRp14P from B10 & pCERF.DRp14
RC   phage from M13mp8 & B10
RC   from pSP64 & phage
RA   Nellen W., Silan C., Firtel R.A.;
RT   "DNA-mediated transformation in Dictyostelium discoideum:
RT   regulated expression of an actin gene fusion";
RL   Mol. Cell. Biol. 4:2890-2898(1984).
RN   [4]
RC   pDd8, pDd9, pDd10 from p2732B & Dictyostelium cysteine proteinase
RC   pDd11.7.10 from p2732B & Dictyostelium cysteine proteinase
RC   pDdG4, pDdG6, pDdG10, pDdG17 from pAT153 & Dictyostelium DNA
RA   Pears C.J., Mahbubani H., Williams J.G.;
RT   "Characterization of two highly diverged but developmentally
RT   co-regulated cysteine proteinase genes in Dictyostelium discoideum";
RL   Nucleic Acids Res. 13:8853-8866(1985).
RN   [5]
RC   pCERF.DRp14 from pBR322 & Tn5 & D.discoideum actin 8 gene
RA   Hirth K.P., Edwards C.A., Firtel R.A.;
RT   "A DNA-mediated transformation system for Dictyostelium discoideum";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7356-7360(1982).
RN   [6]
RC   p2732B from pDdG10
RA   Monahan J.;
RT   ;
RL   Unpublished (1985).
CC   NCBI gi: 209532
CC   NM (pB101TP1)(pB101TP2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Dictyostelium discoideum)
CC   CP ()
CC   FN (cloning)(transformation)
CC   SE ()
CC   PA (Dictyostelium discoideum)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 large SalI-EcoRI
FT                   2. D.discoideum EcoRI-HindIII, actin 6 gene
FT                   3. Tn5 HindIII-SalI, neo gene
FT                   -> plasmid
FT                   1. plasmid BglII
FT                   BAL31 nuclease
FT                   HindIII-HindIII
FT                   Klenow fill in:Klenow fill in
FT                   -> B10
FT                   1. pEMBL18+ remove small EcoRI-BamHI
FT                   2. B10 EcoRI-BamHI, Tn5 neo gene
FT                   -> pB10
FT                   1. pAT153 BamHI
FT                   2. Dictyostelium Sau3A-Sau3A 5000bp
FT                   -> pDdG10
FT                   1. pB10 BamHI
FT                   2. pDdG10 Sau3A-Sau3A 1400bp, CP1 gene 3'
FT                   -> pB10T
FT                   1. pB10T remove small XbaI-HindIII
FT                   2. linker XbaI-NsiI-BamHI-SalI-HindIII
FT                   -> pB10TP1 6952bp
FT                   [XbaI-NsiI-BamHI-SalI-HindIII-KpnI-BglII]
FT   rep_origin      0..0
FT                   /note="ORI Dictyostelium pB101TP"
SQ   Sequence 57 BP; 16 A; 12 C; 13 G; 16 T; 0 other;
     ctagatgcat ggatccatat gaattcgtcg acactagtaa gcttggtacc gagatct