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ID   PBACTMYC   preliminary; circular DNA; SYN; 5620 BP.
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AC   ATCC87060;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Mammal/E.coli plasmid pBact-myc - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBact-myc from pBR322 & c-myc epitope & mammal beta-actin
RA   Cravchik A., Matus A.L.;
RT   "A novel strategy for the immunological tagging of cDNA
RT   constructs";
RL   Gene 137:139-143(1993).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--5.6, PstI--4.8, 0.45, 0.35; EcoRI--5.6. (ATCC staff)
CC   Shuttle expression vector allowing production of a fusion protein
CC   containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can
CC   be used for immunological detection of the recombinant protein in
CC   transfected cells. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Matus A.L.
CC   NM (pBact-myc)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli HB101)(mammalian cells)
CC   CP ()
CC   FN (cloning)(shuttle)(fusion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. c-myc epitope
FT                   3. mammal, beta-actin promoter
FT                   -> pBact-myc"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-NcoI-EcoRI-ClaI-HindIII"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-ClaI"
FT   promoter        0..0
FT                   /note="PRO mammal beta-actin"
FT   terminator      0..0
FT                   /note="TER SV40 polyA"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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