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ID   PBC102K22  preliminary; circular DNA; SYN; 9400 BP.
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AC   ATCC67255;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pBC102-K22 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBC102-K22 from yeast 2 micron & ADH2 gene & TRP1 gene & pBR322
RA   ;
RT   ;
RL   Unpublished (1991).
RL   U.S. Patent No. 5,073,627 dated Dec. 17, 1991.
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RN   [2]
RC   pBC102-K22 from yeast 2 micron & ADH2 gene & TRP1 gene & pBR322
RA   ;
RT   ;
RL   Unpublished (1992).
RL   U.S. Patent No. 5,108,910 dated April 28, 1992.
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CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--9.4; BamHI--uncut; HindIII--6.5, 2.1, 0.8; PstI--5.8, 1.9, 1.7.
CC   (ATCC staff)
CC   Shuttle expression vector utilizing sequences encoding the alpha
CC   factor leader peptide to allow secretion of recombinant proteins. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBC102-K22)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron
FT                   3. yeast, ADH2 gene
FT                   4. yeast, TRP1 gene
FT                   -> pBC102-K22 9400bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO yeast ADH2 gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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