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ID   PBC12BI    preliminary; circular DNA; SYN; 4070 BP.
AC   ATCC67617;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pBC12BI - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pGJ20 from pGJ15 & pBR-F16, FSV LTR
RC   pBC20It from pBR322 & pGJ20, FSV LTR
RC   pBC20Is from pBC20It
RC   pBC21It from pBC20It
RC   pBC21Is from pBC20Is
RC   pBC12BI from pBC12MI & RSV LTR
RC   pKR2/LTR-R from pPB12 & SR-B LTR
RC   pKR2/RAV-0 from pPB12 & RAV-0 LTR
RC   pKR2/FSV from pPB12 & FSV LTR
RA   Cullen B.R., Raymond K., Ju G.;
RT   "Transcriptional activity of avian retroviral long terminal
RT   repeats directly correlates with enhancer activity";
RL   J. Virol. 53:515-521(1985).
RN   [2]
RC   from pBC12BI
RC   from p91023(B)
RA   Cullen B.R.;
RT   "Use of eukaryotic expression technology in the functional
RT   analysis of cloned genes";
RL   Meth. Enzymol. 152:684-704(1987).
RN   [3]
RC   pYM141 from pIN-II series & E.coli lpp gene
RA   Morinaga Y., Franceschini T., Inouye S., Inouye M.;
RT   "Improvement of oligonucleotide-directed site-specific mutagenesis
RT   using double-stranded plasmid DNA";
RL   Biotechnology 2:636-639(1984).
RN   [4]
RC   p91023(B) from pQ2 & mouse DHFR gene
RA   Kaufman R.J.;
RT   "Identification of the components necessary for adenovirus
RT   translational control and their utilization in cDNA expression
RT   vectors";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:689-693(1985).
CC   cDNA clones without an initiation codon may be cloned in frame into
CC   the unique BamHI site 3' to the AUG of the rat preproinsulin gene.
CC   This results in 5 - 6 additional amino terminal amino acids from the
CC   insulin signal peptide. [1]
CC   Genomic sequences are normally inserted between unique HindIII and
CC   SmaI sites (5' to 3').  This deletes the preproinsulin gene from the
CC   vector. [1]
CC   cDNA sequences with a translation initiation codon are usually
CC   inserted between the unique HindIII and BamHI sites (5' to 3'). [1]
CC   Polyadenylation signals (and possibly carboxy terminal amino acids)
CC   are contributed by the preproinsulin gene. [1]
CC   This was constructed from pXF3 (pBR322 minus the poison sequence), a
CC   SV40 ori, and a rat preproinsulin genomic sequence under the control
CC   of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the
CC   BamHI and ClaI sites). [2]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.1; EcoRI--2.8,0.78,0.44; HindIII--4.1; BstEII--4.1. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBC12BI)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli RR1)(vertebrate cells)(E.coli)
CC   HO (monkey COS-1 cell line)(mammal cell line)(avian cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda SRB-td8
FT                   1. ALV SR-B
FT                   -> pLD6
FT                   1. pBR322
FT                   2. lambda SRB-td8
FT                   3. pLD6, ALV SR-B
FT                   -> pBC81
FT                   1. pBC81
FT                   -> pBC81delta3
FT                   1. pBC81delta3
FT                   -> pBC81delta4
FT                   1. pXf3 remove small NruI-SphI
FT                   2. SV40 HindIII-SphI 200bp, SV40 origin
FT                   -> pXF3/ori
FT                   1. pBC81delta4 BglII-BalI, SV40/RSV LTR
FT                   2. pXF3/ori remove small BamHI-ClaI
FT                   :blunt end
FT                   -> pBC12BI"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-HindIII-SmaI-HindIII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO RSV LTR"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;