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ID   PBC12MI    preliminary; circular DNA; SYN; 4100 BP.
AC   ATCC67109;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pBC12MI - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pXF3/ori from pXf3 & SV40, SV40 origin
RC   pBC12MI from pXF3/ori & pBC81delta4, rat preproinsulin II gene
RC   pBC12delta1 from pBC12MI
RC   pBC12/RSV-SEAP from pBC12 & RSV LTR & rat phosphatase gene 3'
RC   pBC12/CMV from pBC12 & CMV LTR
RC   pBC12/RSV/IL2 from pBC12 & RSV LTR & IL-2 gene
RC   pBC12/HIV/IL2 from pBC12 & HIV LTR & IL-2 gene
RA   Cullen B.R.;
RT   "Trans-activation of human immunodeficiency virus occurs via a
RT   bimodal mechanism";
RL   Cell 46:973-982(1986).
RN   [2]
RC   prI2/SV40-46 from pBR322 & SV40 early promoter & rat preproinsulin
RC   pBC8 from pBR322 & lambda SRB-td8 & pLD6, ALV SR-B
RC   pBC81 from pBR322 & lambda SRB-td8 & pLD6, ALV SR-B
RC   pBC81delta1 from pBC81 & linker
RC   pBC81delta2 from pBC81
RC   pBC81delta3 from pBC81
RC   pBC81delta4 from pBC81delta3
RC   pBC81/PA from pBC81 & SV40, polyA
RC   pBC19I from pBR322 & linker & pGJ19, RAV-O LTR
RA   Cullen B.R., Lomedico P.T., Ju G.;
RT   "Transcriptional interference in avian retroviruses -
RT   implications for the promoter insertion model of leukaemogenesis";
RL   Nature 307:241-245(1984).
RN   [3]
RC   lambda HXB-2, lambda HXB-3 from lambda & HTLV-III
RA   Shaw G.M., Hahn B.H., Arya S.K., Groopman J.E., Gallo R.C.,
RA   Wong-Staal F.;
RT   "Molecular charcaterization of human T-cell leukemia
RT   (lymphoytropic) virus type III in the acquired immune deficiency
RT   syndrome";
RL   Science 226:1165-1171(1984).
RN   [4]
RC   plasmid from pUC8 & hCMV
RC   pbetaX1428, pbetaX1429 from plasmid & pbetaX14
RC   C2, C4 from pET-1 & hCMV
RC   pbetaHC22, pbetaHC21 from pbetaX14 & C2
RC   pbetaHC42, pbetaHC41 from pbetaX14 & C4
RC   pJN201 from pCM5027
RC   [pRR23 from hCMV]
RA   Boshart M., Weber F., Jahn G., Dorsch-Hasler K., Fleckenstein B.,
RA   Schaffner W.;
RT   "A very strong enhancer is located upstream of an immediate early
RT   gene of human cytomegalovirus";
RL   Cell 41:521-530(1985).
RN   [5]
RC   pBC12/RSV/CAT from pBC12/RSV/IL2 & pSV2cat
RC   pBC12/HIV/CAT from pBC12/HIV/IL2 & pSV2cat
RC   pBC12/PLAP489 from pBC12/PLAP & oligo
RC   pBC12/SEAP from pBC12/PLAP489 & linker
RC   pBC12/HIV/SEAP from pBC12/HIV/IL2 & pBC12/SEAP
RC   pBC12/RSV/SEAP from pBC12/RSV/IL2 & pBC12/SEAP
RC   pBC12/RSV/SEAP, pBC12/PL/SEAP from pBC12MI
RA   Berger J., Hauber J., Hauber R., Geiger R., Cullen B.R.;
RT   "Secreted placental alkaline phosphatase: a powerful new
RT   quantitative indicator of gene expression in eukaryotic cells";
RL   Gene 66:1-10(1988).
RN   [6]
RC   prI2 from pBR322 & rat preproinsulin II gene
RC   prI2-35 from prI2
RC   prI2-SV40-46 from prI2-35 & pTR405
RC   prI2-SV40-15 from prI2-35 & pTR405
RC   prI2-SV40-XF3 from prI2-35 & pTR405
RA   Lomedico P.T.;
RT   "Use of recombinant DNA technology to program eukaryotic cells to
RT   synthesize rat proinsulin: a rapid expression assay for cloned
RT   genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:5798-5802(1982).
RN   [7]
RC   pCSF-1, pCSF-2, pCSF-3 from p91023(B) & GM-CSF gene
RA   Wong G.G., Witek J.S., Temple P.A., Wilkens K.M., Leary A.C.,
RA   Luxenberg D.P., Jones S.S., Brown E.L., Kay R.M., Orr E.C.;
RT   "Human GM-CSF: molecular cloning of the complementary DNA and
RT   purification of the natural and recombinant proteins";
RL   Science 228:810-815(1985).
RN   [8]
RC   pGJ15 from ASV SR-A env gene
RC   pGJ16 from ASV SR-A env gene & RAV-2 LTR
RC   pGJ18 from ASV SR-A env gene & RAV-0 LTR
RC   pGJ19 from ASV SR-A env gene & ev-2 LTR
RA   Cullen B.R., Skalka A.M., Ju G.;
RT   "Endogenous avian retroviruses contain deficient promoter and
RT   leader sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:2946-2950(1983).
RN   [9]
RC   pBR-F16 from pBR322 & FSV
RA   Shibuya M., Wang L.H., Hanafusa H.;
RT   "Molecular cloning of the Fujinami sarcoma virus genome and its
RT   comparison with sequences of other related transforming viruses";
RL   J. Virol. 42:1007-1016(1982).
RN   [10]
RC   pLD6 from ALV SR-B
RA   Stacey D.;
RT   ;
RL   Unpublished (1984).
RN   [11]
RC   pTR405 from pBR322 & SV40 early region
RA   Roberts T.M., Bikel I., Yocum R.R., Livingston D.M., Ptashne M.;
RT   "Synthesis of simian virus 40 t antigen in Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:5596-5600(1979).
RN   [12]
RC   pBC12/PLAP from pBC12 & PLAP gene
RA   Berger J., Howard A.D., Gerber L., Cullen B.R., Udenfriend S.;
RT   "Expression of active, membrane-bound human placental alkaline
RT   phosphatase by transfected simian cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:4885-4889(1987).
RN   [13]
RC   pCM5027, pCM5028, pCM5029 from CMV immediate early gene promoter
RA   Greenaway P.J., Oram J.D., Downing R.G., Patel K.;
RT   "Human cytomegalovirus DNA: BamHI, EcoRI, and PstI restriction
RT   endonuclease cleavage maps";
RL   Gene 18:355-360(1982).
CC   This was constructed from pXF3 (pBR322 minus the poison sequence), a
CC   SV40 ori, and a rat preproinsulin genomic sequence under the control
CC   of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the
CC   BamHI and ClaI sites).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--2.85, 0.76; HindIII--4.1; BamHI--4.1. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBC12MI)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli MC1061)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pXF3 from pBR322)
CC   BR ()
CC   OF (pBC12deltaI)(pBC12/RSV)(pBC12/HIV)(pBC12/CMV)(pBC12/IL-2)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. ALV SR-B 5' LTR/splice donor
FT                   -> lambda SRB-td8
FT                   1. pBR322
FT                   2. ALV SR-B splice/env/src/3' LTR
FT                   -> pLD6
FT                   1. pBR322 remove small SalI-BamHI
FT                   2. lambda SRBtd8 SalI-BglII, ALV SR-B 5' LTR/splice
FT                   3. pLD6 BglII-BamHI, ALV SR-B splice/env/src/3' LTR
FT                   -> pBC8
FT                   1. pBC8 BstEII, 3' to 3' ALV LTR
FT                   HindIII linker
FT                   2. rat HindIII-HindIII, preproinsulin gene
FT                   -> pBC81
FT                   1. pBC81 remove XhoI-XhoI, 5' ALV LTR/env/src
FT                   -> pBC81delta3
FT                   1. pBC81delta3 remove left end- MstI, ALV except 3'LTR
FT                   -> pBC81delta4
FT                   1. pXf3 remove small NruI-SphI
FT                   2. SV40 HindIII-SphI 200bp, SV40 origin
FT                   -> pXF3/ori
FT                   1. pXF3/ori BamHI-ClaI
FT                   :blunt end
FT                   2. pBC81delta4 BglII-BalI, rat preproinsulin II gene
FT                   -> pBC12MI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO RSV LTR"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;