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ID   PBC16      preliminary; circular DNA; SYN; 4600 BP.
AC   ATCC37338; ATCC37383;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Bacillus plasmid vector pBC16 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBM309 from B.megaterium, megacin
RC   pBM111 from B.megaterium
RC   pBM113 from B.megaterium, megacin
RC   pVT279 from pBM113 & pBC16
RC   pVT54 from pBM113 & pBC16
RC   pVT211, pVT214 from pBM113
RC   pVT525 from pBM113 & pBC16
RC   pVT210 from pBM113 & pBM309
RC   pVT28 from pBM113 & pBM309
RC   pVT26, pVT27 from pBM309
RC   pVT65 from pBM309 & pVT279
RA   Von Tersch M.A., Carlton B.C.;
RT   "Molecular cloning of structural and immunity genes for megacins
RT   A-216 and A-19213 in Bacillus megaterium";
RL   J. Bacteriol. 160:854-859(1984).
RN   [2]
RC   pBC7 from B.cereus DSM 336, bacteriocin gene
RC   pBC16 from B.cereus GP7, tet gene
RC   pBC1, pBC2, pBC3, pBC4, pBC5, pBC6, pBC8, pBC9, pBC10 from B.cereus
RC   pBC11, pBC12, pBC13, pBC14, pBC15, pBC17, pBC18, pBC19 from B.cereus
RC   pBC20, pBC21 from B.cereus
RC   pBS1, pBS2 from B.subtilis
RA   Bernhard K., Schrempf H., Goebel W.;
RT   "Bacteriocin and antibiotic resistance plasmids in Bacillus cereus
RT   and Bacillus subtilis";
RL   J. Bacteriol. 133:897-903(1978).
RN   [3]
RC   phage CP-51 from Bacillus
RC   pBC16 from B.cereus GP7
RC   pC194 from B.thuringiensis 4D11A
RC   from phage CP-51 & pBC16
RC   from phage CP-51 & pC194
RA   Ruhfel R.E., Robillard N.J., Thorne C.B.;
RT   "Interspecies transduction of plasmids among Bacillus anthracis,
RT   B. cereus, and B. thuringiensis";
RL   J. Bacteriol. 157:708-711(1984).
RN   [4]
RC   phage TP-13 from B.thuringiensis
RC   phage YP-18 from B.thuringiensis
RC   phage PBS-1 from B.subtilis
RC   phage Sp-15 from B.subtilis
RA   Barsomian G.D., Robillard N.J., Thorne C.B.;
RT   "Chromosomal mapping of Bacillus thuringiensis by transduction";
RL   J. Bacteriol. 157:746-750(1984).
CC   Plasmid vector for cloning in Bacillus megaterium. [1]
CC   Medium is 3 nutrient agar.
CC   NM (pBC16)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Bacillus megaterium VT1660)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. B.cereus GP7, tet gene
FT                   -> pBC16"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-XbaI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pBC16"
FT   CDS             0..0
FT                   /note="ANT Bacillus streptomycin resistance gene
FT                   (str)"
FT   CDS             0..0
FT                   /note="ANT Bacillus tetracycline resistance gene
FT                   (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;