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ID   PBCL81     preliminary; DNA; SYN; 8100 BP.
AC   ATCC77418;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pBCL8.1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBCL8.1 from pBR322 & LYS2 gene
RA   Lewis B.C., Shah N.P., Braun B.S., Denny C.T.;
RT   "Creation of a yeast artificial chromosome fragmentation vector
RT   based on lysine-2";
RL   Gene Anal. Tech. 9:86-90(1992).
CC   Chromosome acentric fragmentation vector containing LYS2 for use
CC   with artificial chromosomes containing Alu repeats. pBCL8.1 should be
CC   linearized with SalI before transformation into human YAC-containing
CC   yeast strains.  Lys+ Trp+ Ura- colonies should be selected.
CC   Recombinant deletion YACs can be recovered by digestion with BglII,
CC   EcoRI, HindIII, PstI, SacI or XbaI, recircularization, and
CC   transformation into E. coli.
CC   The order of the major features in the plasmid is:  LYS2 - HindIII -
CC   telomere - SalI - Alu repeats - pMB1 ori - ampR - SacI - XbaI - PstI -
CC   EcoRI. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: C.T.Denny
CC   NM (pBCL8.1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(integrating)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast, LYS2 gene
FT                   -> pBCL8.1 8100bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast LYS2 gene"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-PstI-XbaI-SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;