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ID   PBD2       preliminary; circular DNA; SYN; 63 BP.
AC   IG7006;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBD2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBD1 from pUC12 & pUK230
RC   pBD2 from pUC8 & pUK230
RC   pBD3 from pUC13 & pUK230
RC   pBD21 from pBD2 & pMF104, HSV-1 gD gene
RC   pBD5 from pBD3 & pMF100, HSV-1 gD gene
RA   Broker M.;
RT   "Vectors for regulated high-level expression of proteins fused to
RT   truncated forms of Escherichia coli beta-galactosidase";
RL   Gene Anal. Tech. 3:53-57(1986).
RN   [2]
RC   pMF100 from HSV-1 gD gene
RA   Amann E., Broker M., Wurm F.;
RT   "Expression of Herpes simplex virus type 1 glycoprotein C antigens
RT   in Escherichia coli";
RL   Gene 32:203-215(1984).
RN   [3]
RC   pMF104 from HSV-1 gD gene
RA   Broker M., Amann E.;
RT   ;
RL   Appl. Microbiol. Biotechnol. 23/24:0-0(1986).
CC   NM (pBD2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC8 remove EcoRI-SmaI 7bp 231..238,
FT                   \ MCS/2658bp
FT                   2. pUK230 EcoRI-EcoRV 1125bp, lacZ gene
FT                   -> pBD2 3783bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lac gene"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 63 BP; 16 A; 16 C; 18 G; 13 T; 0 other;
     atgaccatga ttacgaattc acaggatggg gatccgtcga cctgcagcca agcttggcac