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ID   PBD20      preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC37102;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pBD20 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBD6 from pSA0501 & pUB110
RC   pBD7 from B.licheniformis DNA & pUB110
RC   pBD8 from pUB110 & pSA2100
RC   pBD9 from pUB110 & pE194
RC   pBD10, pBD11 from pBD8 & pE194
RC   pBD12 from pUB110 & pCM194
RA   Gryczan T.J., Dubnau D.;
RT   "Construction and properties of chimeric plasmids in Bacillus
RT   subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1428-1432(1978).
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CC   A general purpose plasmid vector for cloning in Bacillus subtilis. [3]
CC   Medium is 1179 V Y medium.
CC   NM (pBD20)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBD)
CC   BR (pBD8)(pBD9)(pBD10)(pBD11)(pBD12)(pBD6)(pBD35)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 3728bp, ery gene
FT                   2. pC194 2910bp, cat gene
FT                   -> pBD20 6638bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI-HpaI-PstI-XbaI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pE194"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Bacillus erythromycin resistance gene
FT                   (ery)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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