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ID   PBD35      preliminary; circular DNA; SYN; 8000 BP.
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AC   ATCC37103;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pBD35 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBD35 from pUB110 & pBD15
RC   pBD39 from pBD9
RC   pBD48 from pBD9
RA   Scheer-Abramowitz J., Gryczan T., Dubnau D.;
RT   "Origin and mode of replication of plasmids pE184 and pUB110";
RL   Plasmid 6:67-77(1981).
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CC   A general purpose plasmid vector for cloning in Bacillus subtilis. [2]
CC   Medium is 1179 V Y medium.
CC   NM (pBD35)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBD)
CC   BR (pBD8)(pBD9)(pBD10)(pBD11)(pBD12)(pBD20)(pBD6)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 3728bp
FT                   copy number mutation
FT                   -> pBD15 3500bp
FT                   1. pUB110 XbaI 4548bp 465..465
FT                   2. pBD15 XbaI 3500bp, pE194 3480..3480
FT                   -> pBD35 8050bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-BclI-PstI-BglII-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE194"
FT   CDS             0..0
FT                   /note="ANT Bacillus erythromycin resistance gene
FT                   (erm)"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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