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ID   PBD8       preliminary; circular DNA; SYN; 8900 BP.
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AC   ATCC37097;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pBD8 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBD6 from pSA0501 & pUB110
RC   pBD7 from B.licheniformis DNA & pUB110
RC   pBD8 from pUB110 & pSA2100
RC   pBD9 from pUB110 & pE194
RC   pBD10, pBD11 from pBD8 & pE194
RC   pBD12 from pUB110 & pCM194
RA   Gryczan T.J., Dubnau D.;
RT   "Construction and properties of chimeric plasmids in Bacillus
RT   subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1428-1432(1978).
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CC   A general purpose plasmid vector for cloning in Bacillus subtilis. [2]
CC   Temperature sensitive. [2]
CC   Medium is 1179 V Y medium.
CC   NM (pBD8)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBD)
CC   BR (pBD6)(pBD9)(pBD10)(pBD11)(pBD12)(pBD20)(pBD35)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 XbaI 4548bp 465..465, kan gene
FT                   2. pSA2100 XbaI 7000bp, strep/cat genes
FT                   -> pBD8 8900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-XbaI-EcoRI-HindIII-BglII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Bacillus streptomycin resistance gene
FT                   (str)"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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