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ID   PBETAGIVS1 preliminary; circular DNA; SYN; 503 BP.
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AC   K03428;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pbetaG-IVS1 - incomplete, beta-gal/splicing.
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KW   cloning vector; beta-galactosidase; ribosomal RNA; self-splicing RNA;
KW   exon 1; exon 2; Tetrahymena.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   57-457
RC   pbetaG-IVS1 from pUC8 & pJE457
RA   Price J.V., Cech T.R.;
RT   ;
RL   Unpublished (1985).
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RN   [2]
RP   1-56; 458-503
RC   pJE457 from pIVS11, Tetrahymena rRNA gene
RC   pbetaG-IVS1, pbetaG-IVS13 from pUC8 & pJE457
RC   pbetaGB series from pbetaG-IVS1
RC   pbetaGS series from pbetaG-IVS1
RA   Price J.V., Cech T.R.;
RT   "Coupling of Tetrahymena ribosomal RNA splicing to beta-galactosidase
RT   expression in Escherichia coli";
RL   Science 228:719-722(1985).
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RN   [3]
RP   1-503
RC   pbetaG-IVS1
RA   Price J.V.;
RT   ;
RL   Submitted (04-SEP-1985) by:
RL   Price J.V., University of Colorado, Boulder CO, USA.
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RN   [4]
RC   pTT116 from pBR313 & T. thermophila 26S rRNA gene
RC   pPlac from lacUV5 promoter
RC   pIVS11, pIVS13 from pPlac & pTT116
RA   Kruger K., Grabowski P.J., Zaug A.J., Sands J., Gottschling D.E.,
RA   Cech T.R.;
RT   "Self-splicing RNA: autoexcision and autocyclization of the
RT   ribosomal RNA intervening sequence of Tetrahymena";
RL   Cell 31:147-157(1982).
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RN   [5]
RC   pIVS4 from pIVS11 & linker
RC   pESdelta41-44, pESdelta40-44, pESdelta41-46 from pIVS4
RC   pESdelta35-44, pESdelta36-46, pESdelta39-49 from pIVS4
RC   pESdelta28-58 from pIVS4
RC   pGKdelta225-248, pGKdelta233-246, pGKdelta224-252 from pIVS4
RC   pGKdelta222-243, pGKdelta232-258, pGKdelta218-244 from pIVS4
RC   pJK15, pJK29, pJK39 from pIVS11 & linker
RC   pJK15-SP6 from pSP62-PL1 & pJK15
RC   pJK29-SP6 from pSP62-PL1 & pJK29
RC   pJK43-SP6 from pSP62-PL1 & IVS
RA   Price J.V., Kieft G.L., Kent J.R., Sievers E.L., Cech T.R.;
RT   "Sequence requirements for self-splicing of the Tetrahymena
RT   thermophila pre-ribosomal RNA";
RL   Nucleic Acids Res. 13:1871-1889(1985).
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RN   [6]
RC   pJK43-SP6 from pSP62-PL1 & IVS
RA   Zaug A.;
RT   ;
RL   Unpublished (1985).
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CC   NCBI gi: 209476
CC   NM (pbetaG-IVS1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)(Tetrahymena)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR313 HindIII
FT                   2. T. thermophila HindIII-HindIII 1600bp, 26S rRNA/IVS
FT                   3. T. thermophila HindIII-HindIII 1600bp, 26SrRNA/IVS*
FT                   -> pTT116
FT                   1. lacUV5 promoter
FT                   -> pPlac
FT                   1. pPlac HindIII
FT                   2. pTT116 HindIII-HindIII 1600bp, T. 26S rRNA/IVS
FT                   -> pIVS11 [wild-type IVS]
FT                   1. pIVS11 remove, Tetrahymena rRNA gene exons
FT                   BamHI linker:BamHI linker
FT                   -> pJE457
FT                   1. pUC8 BamHI, MCS
FT                   2. pJE457 BamHI-BamHI 413bp, Tetrahymena rRNA gene IVS
FT                   -> pbetaG-IVS1"
FT   exon            <1..50
FT                   /note="E. coli beta-galactosidase; exon 1"
FT   prim_transcript <1..>503
FT                   /note="E. coli beta-galactosidase mRNA and introns"
FT   misc_recomb     25..26
FT                   /note="pUC8 DNA end/THY DNA start"
FT   intron          51..463
FT                   /note="E. coli beta-galactosidase intron A;
FT                   (no splice consensus); putative"
FT   exon            464..>503
FT                   /note="E. coli beta-galactosidase; exon 1"
FT   misc_recomb     492..493
FT                   /note="THY DNA end/pUC8 DNA start"
FT   CDS             join(1..50,464..>503)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209477"
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SQ   Sequence 503 BP; 144 A; 102 C; 128 G; 129 T; 0 other;
     atgaccatga ttacgaattc ccggggatcg ccgggtaact atgactctct aaatagcaat
     atttaccttt ggagggaaaa gttatcaggc atgcacctgg tagctagtct ttaaaccaat
     agattgcatc ggtttaaaag gcaagaccgt caaattgcgg gaaaggggtc aacagccgtt
     cagtaccaag tctcagggga aactttgaga tggccttgca aagggtatgg taataagctg
     acggacatgg tcctaaccac gcagccaagt cctaagtcaa cagatcttct gttgatatgg
     atgcagttca cagactaaat gtcggtcggg gaagatgtat tcttctcata agatatagtc
     ggacctctcc ttaatgggag ctagcggatg aagtgatgca acactggagc cgctgggaac
     taatttgtat gcgaaagtat attgattagt tttggagtac tcgtaaggta gccaaatgcc
     tcgtcatctc cggatccgtc gac
//