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ID   PBG1       preliminary; circular DNA; SYN; 5700 BP.
AC   ATCC87035;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Schizosaccharomyces/E.coli plasmid pBG1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBG1 from HIS3 gene & pBR322 & ARS
RA   Burke J.D., Gould K.L.;
RT   "Molecular cloning and characterization of the Schizosaccharomyces
RT   pombe his3 gene for use as a selectable marker";
RL   Mol. Gen. Genet. 242:169-176(1994).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2;
CC   PstI--5.7. (ATCC staff)
CC   The his3 insert contains the following restriction sites
CC   (approximate kb from the 5' end):  BamHI--0.49; ClaI--0.04;
CC   EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27;
CC   XhoI--0.84. [1]
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Gould K.L.
CC   NM (pBG1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli HB101)(Schizosaccharomyces pombe)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. pYAC4 BamHI-BamHI 1768bp 4238..6006, HIS3 gene
FT                   3. yeast, ARS
FT                   -> pBG1 5700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   misc_binding    0..0
FT                   /note="MCS SacI-KpnI-SmaI-SalI-PstI-SphI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;