ID   PBG1D      preliminary; circular DNA; SYN; 2305 BP.
XX
AC   L05338;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Desulfovibrio plasmid vector pBG1d - complete.
XX
KW   cloning vector.
XX
OS   Desulfovibrio desulfuricans (strain G100A) DNA
OC   Prokaryotae; Gracilicutes; Scotobacteria;
OC   Sulfate- or sulfur-reducing dissimilatory bacteria.
XX
RN   [1]
RC   pBG1 from Desulfovibrio desulfuricans G100A
RC   pUC4-KIXX [from Pharmacia]
RC   pSC1 from pBG1 & pTZ18U
RC   pSC2 from pSC1 & pUC4-KIXX
RC   pSC26 from pSC2 & pJRD215
RC   pSC3 from pBG1 & pTZ18U
RC   pSC4 from pBG1 & pTZ18U
RC   pSC5 from pSC4 & pUC4-KIXX
RC   pSC56 from pSC5 & pJRD215
RC   pSC7 from pBG1 & pTZ19U
RC   pSC76 from pSC7 & pJRD215
RC   pSC8 from pBG1 & pTZ18U
RC   pSC9 from pSC8 & pUC4-KIXX
RC   pSC96 from pSC9 & pJRD215
RA   Wall J.D., Rapp-Giles B.J., Rousset M.;
RT   "Characterization of a small plasmid from Desulfovibrio
RT   desulfuricans and its use for shuttle vector construction";
RL   J. Bacteriol. 175:4121-4128(1993).
XX
RN   [2]
RC   from Desulfovibrio desulfuricans G100A
RA   Weimer P.J., Van Kavelaar M.J., Michel C.B., Ng T.K.;
RT   "Effect of phosphate on the corrosion of carbon steel and the
RT   composition of corrosion products in two-stage continuous
RT   cultures of Desulfovibrio desulfuricans";
RL   Appl. Environ. Microbiol. 54:386-396(1988).
XX
CC   NM (pBG1d)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Desulfovibrio)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pSC26)(pSC56)(pSC76)(pSC96)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Desulfovibrio desulfuricans G100A
FT                   -> pBG1D"
FT   CDS             complement(1..225)
FT                   /note="GEN Desulfovibrio 5' ORF150; putative;
FT                   linear representation of a circular molecule;
FT                   split ORF150"
FT   repeat_region   292..326
FT                   /note="RPT Group I tandem repeat; putative;
FT                   rpt_unit is 362..372"
FT   repeat_region   396..512
FT                   /note="RPT Group II tandem repeat; putative;
FT                   rpt_unit is 396..416"
FT   CDS             612..1199
FT                   /note="GEN Desulfovibrio ORF195; putative"
FT   CDS             1070..1477
FT                   /note="GEN Desulfovibrio ORF135; putative"
FT   CDS             complement(1097..1585)
FT                   /note="GEN Desulfovibrio ORF162; putative"
FT   repeat_region   1486..1561
FT                   /note="RPT Group III tandem repeat; putative;
FT                   rpt_unit is 1486..1507"
FT   stem_loop       1924..1969
FT                   /note="TER"
FT   CDS             complement(2078..2305)
FT                   /note="GEN Desulfovibrio 3' ORF150; putative;
FT                   linear representation of a circular molecule;
FT                   split ORF150"
XX
SQ   Sequence 2305 BP; 572 A; 597 C; 644 G; 492 T; 0 other;
     cccctcggag ccagcatcgt aatgaccgaa ggccgaagca agcgaagagg tcaagggcag
     aagcgaagcg tcccttgacc ggatgagcgg cgtaggcaga agggaatgga gaggatgact
     acgaagggga attaattccc cttggtggag ttgagtggtt ttaaaaactg gagaggagtc
     catgaaacga aagcagctga ggattgaaac gaagtaacag cgcatactaa ttgtattttt
     tgggtggggg aggagccttg cgacgggcgg cgagcgtagc gaggggcctc tatagcgcgc
     gcatacgcgc gcgcatacgc gcgcgcttct tcttttcttt cttttctctt ttccccctcg
     tttttcgtca gttatttcgg caacctacag acctaaaagt gaccaaaagt acgtctaaag
     gtgaccaaaa gtacgtctaa aggtgaccaa aagtacgtct aaaggtgacc aaaagtacgt
     ggtgtagcct ttgggtgacc aatttgacaa gtggtcacct ttagccataa aggcaaaaga
     acagcccgaa aaggtgcggt aacacctcaa cgggcctaac ctctcacaag ccctaaaatg
     gagggtctgc aatggttgac aggcaattaa ccttacccgg cgagatagtc aaaaagtcaa
     acgcactggc tagggccaag tggtccccta agtccgtgtg ggaaccgcgc atggtcgcgc
     tggtagcttc catggtccgt caggacgatg cggactttca ggaataccgc tttcctgtct
     ccaccctttg cgatgagcaa gagctatccg gcaagaggta caaggacatt aaggccgctg
     tagaaaatgt gatgaaagcc tatgtgatga ttgaggaagg caaaaattac gtggcgtata
     gcatcttctc aaagtgtggc tatgaaaacg gtgtcatggt cgccgccttc catcccgatc
     tgaaacccca ttttctcggc cttcaaaagc agttcacaag gtatagcctc atggaatttc
     tggtgctgcc tagcacctac agccaaaggg ttttcgaggt tttgaggtcg tgggacgaca
     agccggaagt gaccattcac ttgctgatct tcatgaaatg gtgggtacgc ctccttctct
     gcggaagaag taccccgact ttcgccgtaa ggttcttgag aaggcccaca aggacataaa
     ccgccatacc gcgcttgctt acgagtggga agccgtgaag aacgggcgct cggtcgcggg
     tatccgcttc ctgttcgcgc agaagcgggc gggcagggtc gtagccaaga aacaggccaa
     ggtcgtgcag gacagcagcg acgccaagaa caaggccttc atcgccgcga ccaagtgcca
     cgcggccagc aagggccgct gcacgccgaa agacaacctc cagtgccaag tgtgcgccca
     gctcgtcagc ctgggcaaga gagccggaac agcctaaacc tcttttgtag caccccccct
     ttgaggctgt agcacccccc ttttgaggct gtagcacctt gtgacgtgcc tgtagcacct
     tttcagactt cacattttta accataacgt gctgttttaa cagaaatttt ctttacgttt
     cagcaccggc tgatctcgct tttttgtgcc acaaaaaggc tatcctgcca agcggcatta
     aaacaagcca cctcatcaac aaaatgaagg ggggcgcggc ccgctcacat ggttgcaggt
     gaagaagatt ggaaatcgag gcgaaggtta ttccattccc cgcagtagaa cctgaacagg
     gaagcctgtt ctaatagccc taaaatcgat tttaagcgca ggaacaagca agaagagact
     caggctcacc gagggaaatt tcctccctta cgcccccttc tacgcttcgt tatcgctacg
     ccgaaaaaaa gagcaaggga cgcttcgctt ctgcccttgc tctttttttg cgccttcgct
     gcactccgct cacgaaggcg gctccggggg aaatttcagc ctggttccaa cgacaagcga
     agcgggatct gcggggggcc cccgtgaggg ggggcggcta gccccatccc ctgcttgcag
     gggcaggacc ggcgtagccg gagcgagtgg tgatggtctt gtgatcgtcc agagtgattt
     ccgttgtgct ggactctccc gattctcgac gagctgaaag cgagaagagc gcacttatac
     atcgctgcgc gacgtgtgcg ccctgcgggg ctaagtcatc ggaacaaaag agtttccccg
     tagtggggaa aagggggctt gaatt
//