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ID   PBGP120    preliminary; circular DNA; SYN; 17000 BP.
AC   ATCC39055;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pBGP120 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   from pBGP120
RA   O'Farrell P., Polisky B., Gelfand D.H.;
RT   "Regulated expression by readthrough translation from a
RT   plasmid-encoded beta-galactosidase";
RL   J. Bacteriol. 134:645-654(1978).
RN   [2]
RC   pBGP100, pBGP101 from RSF2124 & lambda plac5
RC   pBGP120 from pBGP100
RC   pBGP123 from pBGP120 & frog 28S gene
RC   pBGP124 from pBGP120 & frog 28S gene
RA   Polisky B., Bishop R.J., Gelfand D.H.;
RT   "A plasmid cloning vehicle allowing regulated expression of
RT   eukaryotic DNA in bacteria";
RL   Proc. Natl. Acad. Sci. U.S.A. 73:3900-3904(1976).
RN   [3]
RC   lambda plac5 from lambda & lac operator
RC   phi80 plac1 from phage phi80 & lac operator
RA   Shapiro J., Machattie L., Eron L., Ihler G., Ippen K., Beckwith J.;
RT   "Isolation of pure lac operon DNA";
RL   Nature 224:768-774(1969).
RN   [4]
RC   pOP1 from pBGP120
RC   pOP1delta6 from pOP1
RA   Gelfand D.H., Shepard H.M., O'Farrell P.H., Polisky B.;
RT   "Isolation and characterization of ColE1-derived plasmid
RT   copy-number mutant";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:5869-5873(1978).
RN   [5]
RC   from RSF1030
RC   from RSF1050
RC   from ColE1
RC   from pFH118
RA   Conrad S.E., Campbell J.L.;
RT   "Role of plasmid-coded RNA and ribonuclease III in plasmid DNA
RT   replication";
RL   Cell 18:61-71(1979).
RN   [6]
RC   PS001 from pOP1delta6 & pBGP120
RC   from pOP1delta6 & pSC101
RC   pNOP1 from pOP1delta6
RC   pNOP10 from pOP1delta6 & pBGP120
RC   pNOP11, pNOP12 from pNOP10
RA   Shepard H.M., Gelfand D.H., Polisky B.;
RT   "Analysis of a recessive plasmid copy number mutant: evidence for
RT   negative control of Col E1 replication";
RL   Cell 18:267-275(1979).
RN   [7]
RC   pOP42-I, pOP42-II from pOP1delta6
RC   pNOP42, pNOP42-I, pNOP42-II from pNOP1
RC   pOP36 from pOP42-I
RC   pOP136 from pOP42-II
RC   pNOP36 from pNOP42-I
RC   pNOP136 from pNOP42-II
RA   Muesing M., Tamm J., Shepard H.M., Polisky B.;
RT   "A single base-pair alteration is responsible for the DNA
RT   overproduction phenotype of a plasmid copy-number mutant";
RL   Cell 24:235-242(1981).
CC   A multicopy plasmid cloning vector permitting controlled expression of
CC   inserted DNA as a fusion protein.
CC   Contains the lac promoter and operator and a major portion of the lacZ
CC   gene.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBGP120)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DG75)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. F lac temperature sensitive
FT                   transposition
FT                   2. E. coli chromosome, near lambda att site
FT                   -> E. coli EC2710
FT                   1. E. coli EC2710 remove from i gene to lambda
FT                   -> E. coli modified
FT                   1. E. coli modified 4000bp, lacZ/lacOP
FT                   transposition
FT                   2. lambda remove 4000bp, 5' to EcoRI2 to 5' to EcoRI3
FT                   -> lambda plac5
FT                   1. RSF2124 EcoRI
FT                   2. lambda plac5 EcoRI-EcoRI 4400bp, lacZ/lacOP/lambda
FT                   -> pBGP100 
FT                   1. pBGP100 EcoRI, lambda/RSF2124
FT                   lambda 5' exonuclease
FT                   -> pBGP120 [unique EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;