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ID   PBGRF      preliminary; circular DNA; SYN; 167 BP.
XX
AC   IG8040;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E. coli plasmid vector pbGRF - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pbGRF from pUC19 & oligos
RC   pWAP.GRF from pbGRF & WAP promoter
RC   pMMTV.GRF from pbGRF & MMTV, MMTV promoter
RC   pBLV.WAP.GRF from pWAP.GRF & pBLV-913, BLV
RC   pBLV.MMTV.GRF from pMMTV.GRF & pBLV-913, BLV
RA   Mehigh C.S., Elias V.D., Mehigh R.J., Helferich W.G., Tucker H.A.;
RT   "Development of a recombinant bovine leukemia virus vector for
RT   delivery of a synthetic bovine growth hormone-releasing factor
RT   gene into bovine cells";
RL   J. Anim. Sci. 71:687-693(1993).
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RN   [2]
RC   pBL-H2neo from pSV2neo & BLV LTR
RC   RD-4.4 from pBLV-913 & pBL-H2neo
RC   BLV-SVNEO from BLV & SV40 early promoter/neo gene
RC   pBLV-SVNEO from pBLV-913 & SV40 early promoter/neo gene
RA   Derse D., Martarano L.;
RT   "Construction of a recombinant bovine leukemia virus vector for
RT   analysis of infectivity";
RL   J. Virol. 64:401-405(1990).
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RN   [3]
RC   pBLV-913 from BLV
RA   Derse D.;
RT   "trans-acting regulation of bovine leukemia virus mRNA processing";
RL   J. Virol. 62:1115-1119(1988).
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CC   NM (pbGRF)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(bovine cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 EcoRI
FT                   2. oligo [see sequence]
FT                   -> pbGRF [3' EcoRI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 167 BP; 46 A; 39 C; 38 G; 44 T; 0 other;
     aattgatgta cgctgacgct atcttcacta actcttaccg taaagttctg ggtcaactgt
     ctgctcgtaa actgctgcaa gacatcatga accgtcagca gggtgaacgt aaccaggaac
     agggtgctaa agttcgtctg tcttagtaac gcgcgttctc aataaag
//