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ID   PBGS18M    preliminary; circular DNA; SYN; 4400 BP.
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AC   M18327; M18328; M18329; ATCC37457;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBGS18- - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPH120 from pHP34 & pLG338
RC   pPH121 from pPH120
RC   pPH124 from pPH121
RC   pPH125 from pPH124 & pPH120
RC   pPH126 from pPH125
RC   pPH114 from pPH125
RC   pBS105 from pPH114
RC   pteH8 from pPH125 & pUC8
RC   pBS8 or pBGS8 from pteH8 & pBS105
RC   pBS8+ or pBGS8+, pBS8-  or pBGS8- from pBS8 & pEMBL8+
RC   pteH9 from pPH125 & pUC9
RC   pBS9 from pteH9 & pBS105
RC   pBS9+, pBS9- from pBS9 & pEMBL9+
RC   [pBG131 from pBS series]
RC   [pBGS18 from pUC18]
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT   pUC9, pEMBL8 and pEMBL9";
RL   Gene 41:337-342(1986).
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CC   The duplicate HindIII, SmaI and AccI sites have been removed.
CC   Kanamycin-resistant analog of EMBL18.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pBGS18-)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pEMBL18- remove, 3960bp-
FT                   2. E. coli HindIII 1000bp, kan gene
FT                   fill in
FT                   SmaI 1000bp, kan gene
FT                   fill in
FT                   AccI 1000bp, kan gene
FT                   fill in
FT                   E. coli 1000bp, kan gene
FT                   -> pBGS18- 4400bp [unique HindIII/SmaI/AccI]"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   AccI-HincII-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SstI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-AccI-HincII-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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