back Return to this vector's summary.
ID   PBGS8      preliminary; circular DNA; SYN; 3660 BP.
AC   M18327; M18328; M18329; ATCC37449;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBGS8 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pPH120 from pHP34 & pLG338
RC   pPH121 from pPH120
RC   pPH124 from pPH121
RC   pPH125 from pPH124 & pPH120
RC   pPH126 from pPH125
RC   pPH114 from pPH125
RC   pBS105 from pPH114
RC   pteH8 from pPH125 & pUC8
RC   pBS8 or pBGS8 from pteH8 & pBS105
RC   pBS8+ or pBGS8+, pBS8-  or pBGS8- from pBS8 & pEMBL8+
RC   pteH9 from pPH125 & pUC9
RC   pBS9 from pteH9 & pBS105
RC   pBS9+, pBS9- from pBS9 & pEMBL9+
RC   [pBG131 from pBS series]
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT   pUC9, pEMBL8 and pEMBL9";
RL   Gene 41:337-342(1986).
CC   Kanamycin-resistant analog of pUC8.
CC   Duplicate HindIII, SmaI and AccI sites have been removed.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pBGS8)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pPH125 SmaI 5300bp, pHP34 4299..4299
FT                   fill in
FT                   remove EcoRI-BamHI 1300bp, pHP34 4292..5300..306
FT                   \ 4000bp
FT                   2. E. coli EcoRI-BamHI 2600bp, pbpB gene
FT                   -> pPH114 6600bp [unique HindIII in kan]
FT                   1. pPH114 HindIII 6600bp, kan gene
FT                   mutagenesis of ends
FT                   -> pBS105 6600bp [no SmaI, no HindIII]
FT                   1. pPH125 remove PvuII-EcoRI 2293bp 1999..4292,
FT                   \ tet gene/3000bp
FT                   S1 nuclease
FT                   2. pUC8 HaeII-HaeII 794bp 2300..2665..429, lacPOZ'
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pteH8 3660bp
FT                   1. pteH8 remove MluI-MluI 800bp, kan gene
FT                   2. pBS105 MluI-MluI 800bp,
FT                   \ kan gene with no SmaI or HindIII
FT                   -> pBGS8 3660bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-AccI-HincII-BamHI-SmaI-
FT                   EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-AccI-HincII-
FT                   BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;