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ID   PBGS8M     preliminary; circular DNA; SYN; 4400 BP.
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AC   M18327; M18328; M18329; ATCC37451;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBGS8- - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPH120 from pHP34 & pLG338
RC   pPH121 from pPH120
RC   pPH124 from pPH121
RC   pPH125 from pPH124 & pPH120
RC   pPH126 from pPH125
RC   pPH114 from pPH125
RC   pBS105 from pPH114
RC   pteH8 from pPH125 & pUC8
RC   pBS8 or pBGS8 from pteH8 & pBS105
RC   pBS8+ or pBGS8+, pBS8-  or pBGS8- from pBS8 & pEMBL8+
RC   pteH9 from pPH125 & pUC9
RC   pBS9 from pteH9 & pBS105
RC   pBS9+, pBS9- from pBS9 & pEMBL9+
RC   [pBG131 from pBS series]
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT   pUC9, pEMBL8 and pEMBL9";
RL   Gene 41:337-342(1986).
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CC   The duplicate HindIII, SmaI and AccI sites have been removed.
CC   Kanamycin-resistant analog of EMBL8.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pBGS8-)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBGS8 remove small AhaIII-AhaIII, 3646bp
FT                   2. pEMBL8- SspI-SspI 711bp 914..1625, f1 ori
FT                   -> pBGS8- 4357bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-AccI-HincII-BamHI-SmaI-
FT                   EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-AccI-HincII-
FT                   BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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