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ID   PBGST7     preliminary; circular DNA; SYN; 75 BP.
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AC   M14748;
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DT   15-DEC-1988 (Rel. 3, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pBGST7 - incomplete, lacZ region.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-75
RC   pUT718 from pUC18 & pT7-2, T7 promoter
RC   pBGST7 from pUT718 & pJE457
RA   Been M.D., Cech T.R.;
RT   "One binding site determines sequence specificity of Tetrahymena
RT   pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity";
RL   Cell 47:207-216(1986).
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CC   NCBI gi: 208703
CC   NM (pBGST7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)(T7 promoter)(Tetrahymena pre-rRNA intervening sequence)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 HaeII
FT                   T4 DNA polymerase, blunt ends
FT                   calf intestinal phosphatase
FT                   2. pT7-2 PvuII-HindIII, T7 promoter
FT                   T4 DNA polymerase, blunt ends
FT                   -> pUT718
FT                   1. pUT718 remove small KpnI-SphI, MCS
FT                   T4 DNA polymerase, blunt ends
FT                   calf intestinal phosphatase
FT                   2. pJE457 BamHI-BamHI, IVS
FT                   S1 nuclease
FT                   -> pBGST7"
FT   misc_feature    0..0
FT                   /note="pT7-2 PvuII-HindIII, blunt-ended (T7 promoter),
FT                   inserted into pUC18 HaeII 680 (-230 to MCS),
FT                   to make pUT718;
FT                   pJE457 KpnI-SphI (Tetrahymena pre-rRNA intervening
FT                   sequence with short exons) blunt-ended,
FT                   replaces pUC18 KpnI-SphI (MCS), to make pBGST7"
FT   CDS             1..>75
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 208704"
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SQ   Sequence 75 BP; 21 A; 18 C; 16 G; 20 T; 0 other;
     atgaccatga ttacgaattc gagctcgggt aactatgact ctcttaaggt agccaaatgc
     ctcgtcacaa gcttg
//