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ID   PBI121     preliminary; DNA; SYN; 1 BP.
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AC   IG1037; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBI121 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RC   pBI121 from pBI101.1 & CMV 35S promoter
RC   pBI131 from pBI101.1 & tobacco rbcS promoter
RC   pROK1 from CMV 35S promoter
RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile gene
RT   fusion marker in higher plants";
RL   EMBO J. 6:3901-3907(1987).
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CC   NM (pBI121)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(plant)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pBI101)
CC   BR (pBI221)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. CMV, 35S promoter
FT                   -> pROK1
FT                   1. pBI101 remove HindIII-BamHI 30bp 2499..2529,
FT                   \ MCS 5320bp
FT                   2. pROK1 HindIII-BamHI 200bp, CMV 35S promoter
FT                   -> pBI121 5500bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO Agrobacterium Ti plasmid nopaline synthase"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-CaMV 35S promoter-
FT                   XbaI-SmaI"
FT   promoter        0..0
FT                   /note="PRO Cauliflower mosaic virus (CaMV) 35S gene"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT SnoBI"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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