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ID   PBI221     preliminary; circular DNA; SYN; 1 BP.
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AC   IG1038; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBI221 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pUC19 & oligo, translation initiator
RC   pRAJ275 from pRAJ255 & plasmid
RC   pBI221.1 from pBI121 & pUC19
RC   pBI121.2 from pRAJ275 & pBIN19
RC   pBI221.2 from pRAJ275 & pUC19
RA   Jefferson R.A.;
RT   "Assaying chimeric genes in plants: the GUS gene fusion system";
RL   Plant Mol. Biol. Rep. 5:387-405(1987).
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CC   NM (pBI221)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pBI121)(pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBI121 HindIII-EcoRI 2400bp,
FT                   \ 2499..-..2529..4687/35S/GUS/nos pBI101 2499..4687
FT                   2. pUC19 remove EcoRI-HindIII 51bp 397..448,
FT                   \ MCS/2635bp
FT                   -> pBI221.1 5000bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-CaMV 35S promoter-
FT                   XbaI-SmaI"
FT   promoter        0..0
FT                   /note="PRO Cauliflower mosaic virus (CaMV) 35S gene"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT SnoBI"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti nopaline synthase gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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