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ID   PBIIHI2    preliminary; circular DNA; SYN; 5200 BP.
AC   ATCC67902;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBIIHI.2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBIIHI.2 from pNO1523 & linker
RA   ;
RT   ;
RL   Unpublished (1991).
RL   U.S. Patent No. 5,015,581 dated May, 14, 1991.
CC   Permits positive selection for inserts,
CC   based on the dominance of the wild-type rpsL (ribosomal protein S12)
CC   gene in the plasmid.  The SmaI and one HpaI site are in this gene.
CC   (ATCC staff)
CC   Derived from pNO1523 by inserting a BglII linker at the EcoRI site and
CC   a HpaI linker at the PvuII site. (personal communication)
CC   The order of the major features in this plasmid is:EcoRI - BglII -
CC   EcoRI - ClaI - HindIII - BamHI - rpsL - ori - bla. (personal
CC   communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--5.2; BglII--5.2; HindIII--5.2; PstI--3.6, 1.6. (ATCC staff)
CC   Medium is 1315 1065 plus ampicillin (100 ug/ul).
CC   NM (pBIIHI.2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli RR1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pNO1523 ?EcoRI 4799bp
FT                   BglII linker 6bp agatct
FT                   PvuII 4799bp 2505..2505
FT                   HpaI linker 6bp gttaac
FT                   -> pBIIHI.2 4799bp [5200bp]
FT                   \ [SmaI in rpsL+, ori/amp/str prom/rpsL+]"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII-ClaI-HindIII-EcoRV-SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli str gene"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
FT   CDS             0..0
FT                   /note="GEN E. coli rpsL+ reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;