back Return to this vector's summary.
ID   PBIIHI2KI  preliminary; circular DNA; SYN; 1 BP.
XX
AC   ATCC68002;
XX
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pBIIHI.2KI - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBIIHI.2KI from pNO1523 & linker
RA   ;
RT   ;
RL   Unpublished (1993).
XX
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--3.8, 1.55; EcoRI--5.6; HindIII--5.6; HpaI--3.6, 1.9.(ATCC staff)
CC   The vector is derived from pNO1523 (ATCC 37095) by the insertion of a
CC   BglII linker at the EcoRI site, a HpaI linker at the PvuII site, and a
CC   KpnI linker at the EcoRV site. (personal communication)
CC   Note: This material is cited in a U.S and/or other Patent and may not
CC   be used to infringe the patent claims.
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   NM (pBIIHI.2KI)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)(NEB)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pNO1523 ?EcoRI 4799bp
FT                   BglII linker 6bp agatct
FT                   PvuII 4799bp 804..804, could be 2505..2505
FT                   HpaI linker 6bp gttaac
FT                   EcoRV 4799bp 118..118
FT                   KpnI linker 6bp ggtacc
FT                   -> pBIIHI.2KI 4799bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli strep gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//