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ID   PBKRSV     preliminary; circular DNA; SYN; 1 BP.
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AC   IG2052;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBK-RSV - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBK-RSV from pBR322 & RSV
RC   pBK-CMV from pBR322 & CMV
RC   lambda ZAP Express from lambda & pBK-CMV
RA   Alting-Mees M.A.;
RT   ;
RL   Strategies 5:58-61(1992).
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CC   NM (pBK-RSV)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli XL-1-Blue MRF')
CC   CP ()
CC   FN (cloning)(cloning cDNA)(cloning unidirectional)(sequencing)
CC   FN (expression prokaryote)(expression eukaryote)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR (pBK-CMV)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. RSV, immediate-early promoter
FT                   3. E. coli, lacZ gene/lacUV5 promoter
FT                   4. T3, promoter
FT                   5. T7, promoter
FT                   6. SV40, splice/polyA
FT                   7. f1, ori
FT                   8. Tn5, neo gene
FT                   9. SV40, ori/tk gene
FT                   -> pBK-RSV"
FT   misc_binding    0..0
FT                   /note="SIT NsiI"
FT   promoter        complement(0..0)
FT                   /note="PRO RSV immediate-early"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS SacI-EcoRI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT SV40 5' splice site"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT SV40 3' splice site"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 polyA site"
FT   misc_binding    0..0
FT                   /note="SIT MluI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1-"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_feature    0..0
FT                   /note="TER helper phage initiator"
FT   misc_feature    0..0
FT                   /note="TER helper phage terminator"
FT   misc_binding    0..0
FT                   /note="SIT NaeI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_binding    0..0
FT                   /note="SIT MstII"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   polyA_signal    0..0
FT                   /note="PLA mammal thymidine kinase polyA site"
FT   misc_binding    0..0
FT                   /note="SIT MstII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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