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ID PBLA11 preliminary; circular DNA; SYN; 1 BP.
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE E. coli plasmid vector pBLA 11 - incomplete.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pBLA 11 from pBR322 & lambda, pL
RL Unpublished (1988).
RL U.S. Patent No. 4,742,004 dated May 3, 1988.
CC Restriction digests of the clone give the following sizes (kb):
CC EcoRI--3.8; HindIII--3.8; SalI--3.8. (ATCC staff)
CC This contains the ribosomal binding site of the beta-lacatamase gene.
CC Medium is 1273 LB plus tetracycline.
CC NM (pBLA 11)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/tet gene
FT 2. lambda, pL
FT -> pBLA 11"
FT promoter 0..0
FT /note="PRO bacteriophage lambda pL"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;