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ID   PBM2240    preliminary; circular DNA; SYN; 7250 BP.
AC   ATCC87021;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli phagemid vector pBM2240 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBM2906 from pRS series
RC   pBM2240 from pRS316
RC   pBM2384 from pRS426
RA   Erickson J.R., Johnston M.N.;
RT   "Direct cloning of yeast genes from an ordered set of lambda
RT   clones in Saccharomyces cerevisiae by recombination in vivo";
RL   Genetics 134:151-157(1993).
CC   YC-type shuttle vector designed to clone yeast genes that are
CC   difficult to clone by rescuing inserts from the vector lambdaMG3 by
CC   homologous recombination.
CC   S. cerevisiae is cotransformed with the lambda clone of interest and
CC   vector linearized with an EcoRI/XhoI double digest.
CC   Ura+ colonies are tested for complementation of a mutant phenotype.
CC   Constructed by insertion of a 0.858 kb BamHI-XbaI fragment
CC   and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites
CC   of pRS316 (ATCC 77145).
CC   The order of the major features in this plasmid is: f1 ori - lacZ -
CC   T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI -
CC   T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: M.N.Johnston
CC   NM (pBM2240)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda MG3
FT                   1. pRS316 KpnI 4887bp 2100..2100, MCS
FT                   2. lambda MG3 KpnI-KpnI 1503bp, lambda 17058..18561
FT                   -> plasmid 6390bp
FT                   1. plasmid remove XbaI-BamHI 12bp 2018..2030,
FT                   \ MCS 6378bp
FT                   2. lambda MG3 BamHI-XbaI 858bp
FT                   -> pBM2240 7246bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-PstI-
FT                   SmaI"
FT   misc_feature    0..0
FT                   /note="centromere CEN6"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast CEN6/ARS4"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;