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ID   PBM2384    preliminary; DNA; SYN; 8100 BP.
AC   ATCC87020;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli phagemid vector pBM2384 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBM2906 from pRS series
RC   pBM2240 from pRS316
RC   pBM2384 from pRS426
RA   Erickson J.R., Johnston M.N.;
RT   "Direct cloning of yeast genes from an ordered set of lambda
RT   clones in Saccharomyces cerevisiae by recombination in vivo";
RL   Genetics 134:151-157(1993).
CC   YE-type shuttle vector designed to clone yeast genes that are
CC   difficult to clone by rescuing inserts from the vector lambdaMG3 by
CC   homologous recombination.
CC   S. cerevisiae is cotransformed with the lambda clone of interest and
CC   vector linearized with an EcoRI/XhoI double digest.
CC   Ura+ colonies are tested for complementation of a mutant phenotype.
CC   Constructed by inserting a 0.858 kb BamHI-XbaI fragment and a
CC   1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of
CC   pRS426 (ATCC 77107).
CC   The order of the major features in this plasmid is:
CC   ori(f1) - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS -
CC   BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - 2 micron ori -
CC   URA3. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: M.N.Johnston
CC   NM (pBM2384)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda MG3
FT                   1. pRS426 KpnI 5726bp 2009..2009, MCS
FT                   2. lambda MG3 KpnI-KpnI 1503bp, lambda 17058..18561
FT                   -> plasmid 7229bp
FT                   1. plasmid remove BamHI-XbaI 12bp 2071..2083,
FT                   \ MCS 7217bp
FT                   2. lambda MG3 BamHI-XbaI 858bp
FT                   -> pBM2384 8075bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   PstI-SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="centromere CEN6"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;