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ID   PBM2906    preliminary; DNA; SYN; 7360 BP.
AC   ATCC87022;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli phagemid vector pBM2906 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBM2906 from pRS series
RC   pBM2240 from pRS316
RC   pBM2384 from pRS426
RA   Erickson J.R., Johnston M.N.;
RT   "Direct cloning of yeast genes from an ordered set of lambda
RT   clones in Saccharomyces cerevisiae by recombination in vivo";
RL   Genetics 134:151-157(1993).
CC   S. cerevisiae is cotransformed with the lambda clone of interest
CC   and vector linearized with an EcoRI/XhoI double digest.
CC   Ura+ colonies are tested for complementation of a mutant phenotype.
CC   The order of the major features in the plasmid is: f1 ori - lacZ -
CC   T7 promoter - KpnI/lambdaMG14/KpnI - MCS - BamHI/lambdaMG14/XbaI -
CC   T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: M.N.Johnston
CC   NM (pBM2906)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda MG14
FT                   1. pRS426 KpnI 5726bp 2009..2009, MCS
FT                   2. lambda MG14 KpnI-KpnI 1503bp, lambda 17058..18561
FT                   -> plasmid 7229bp
FT                   1. plasmid remove BamHI-XbaI 12bp 2071..2083,
FT                   \ MCS 6500bp
FT                   2. lambda MG14 BamHI-XbaI 858bp
FT                   -> pBM2906 7360bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_feature    0..0
FT                   /note="centromere CEN6"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast CEN6/ARS4"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;