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ID   PBML       preliminary; circular DNA; SYN; 95 BP.
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AC   X56013; X56014;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBM(L) - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pT7T3 from pTZ18U
RC   pBM(L), pBM(S) from pT7T3 & oligo
RC   NOTE: pT7T3 [Pharmacia]
RA   Waye M.M., Mui F., Hodge K., Li V.;
RT   "A phagemid vector library for cloning DNA with four-nucleotide 5'
RT   or 3' overhangs";
RL   Plasmid 26:74-77(1991).
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CC   MCS. oligonucleotide linker.
CC   NM (pBM L)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pT7T3 from pTZ18U)
CC   BR (pBM S)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ18U 2860bp
FT                   -> pT7T3 2860bp
FT                   1. pT7T3 remove SalI-HindIII 18bp 289..307, MCS 2840bp
FT                   2. oligo HindIII-BstXI-BspMI-SalI 24bp
FT                   \ tcgaacctgcccaannnnttggca
FT                   -> pBM(L) 2860bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 95 BP; 25 A; 22 C; 21 G; 23 T; 4 other;
     atgattacga atttaatacg actcactata gggaattcga gctcggtacc cggggatcct
     ctagagtcga acctgcccaa nnnnttggca agctt
//