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ID   PBP103     preliminary; circular DNA; SYN; 6200 BP.
AC   ATCC77087;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pBP103 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   plasmid from YTCA-1x & pSP73
RC   pBP103 from plasmid & pRS303
RC   pBP108, pBP109 from pBP103 & BLUR8
RC   pBP110, pBP111 from pBP103 & pL1.1A
RC   pBP81 from pBP63A & YTCA-1x
RA   Pavan W.J., Hieter P., Sears D., Burkhoff A., Reeves R.H.;
RT   "High-efficiency yeast artificial chromosome fragmentation
RT   vectors";
RL   Gene 106:125-127(1991).
CC   Generic chromosome fragmentation vector containing promoters for in
CC   vitro RNA synthesis.  The unique cloning sites can be used to insert
CC   targeting sequences, such as cDNAs. [1]
CC   Any unique restriction site between the targeting sequence and the
CC   telomere-adjacent sequence can be used to linearize the plasmid before
CC   transformation. [1]
CC   The EcoRV and ClaI sites between the SP6 promoter and the telomere
CC   sequence can be used for recovery from deletion derivatives of YAC
CC   insert sequences adjacent to the fragmentation site. [1]
CC   A 0.24 kb EcoRI/SalI fragment containing the telomere-adjacent
CC   sequence from YTCA-1x was ligated into the MCS of pSP73.  The EcoRI
CC   and BamHI sites of the MCS were destroyed and a 0.3 kb NdeI/PvuII
CC   fragment was inserted into the PvuII site of pRS303. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.6; EcoRI--4.6; SalI--4.6; SalI/EcoRI--4.4, 0.3. (ATCC staff)
CC   The order of the major features in this plasmid is: bla - HIS3 -
CC   lacZ'/MCS - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV
CC   - SP6 promoter. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBP103)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH252)
CC   HO (Saccharomyces cerevisiae CGY2516 [ATCC 74013])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast 240bp, telomere
FT                   -> YTCA-1x 4600bp
FT                   1. YTCA-1x EcoRI-SalI 240bp, telomere
FT                   2. pSP73 remove EcoRI-SalI 33bp 25..58, MCS 2431bp
FT                   -> plasmid 2670bp [no BamHI, no EcoRI]
FT                   1. plasmid NdeI-PvuII 373bp,
FT                   \ pSP73 166bp 2382..2464..84
FT                   2. pRS303 PvuII 4443bp 1953..1953, may be 2398..2398
FT                   -> pBP103 6200bp [4600bp]"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SacII-BstXI-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-
FT                   EcoRI-EcoRV-HindIII-ClaI-SalI-XhoI-ApaI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SacII-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-EcoRI-XhoI-ApaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FFT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;