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ID   PBP109     preliminary; circular DNA; SYN; 4900 BP.
AC   ATCC77089;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pBP109 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   plasmid from YTCA-1x & pSP73
RC   pBP103 from plasmid & pRS303
RC   pBP108, pBP109 from pBP103 & BLUR8
RC   pBP110, pBP111 from pBP103 & pL1.1A
RC   pBP81 from pBP63A & YTCA-1x
RA   Pavan W.J., Hieter P., Sears D., Burkhoff A., Reeves R.H.;
RT   "High-efficiency yeast artificial chromosome fragmentation
RT   vectors";
RL   Gene 106:125-127(1991).
CC   Acentric chromosome fragmentation vector targeting Alu sequences.
CC   Contains promoters for in vitro RNA synthesis. [1]
CC   Constructed from pBP103 (ATCC 77087) by inserting a 0.3 kb
CC   Alu-containing sequence from BLUR8 into the BamHI site.  pBP108
CC   (ATCC 77088) and pBP109 (ATCC 77089) differ in the orientation of the
CC   Alu sequence. [1]
CC   Any unique restriction site between the targeting sequence and the
CC   telomere-adjacent sequence can be used to linearize the plasmid before
CC   transformation. [1]
CC   The EcoRV and ClaI sites between the SP6 promoter and the telomere
CC   sequence can be used for recovery from deletion derivatives of YAC
CC   insert sequences adjacent to the fragmentation site. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.6,0.3; EcoRI--4.9; SmaI--4.9; PstI--3.4, 1.15, 0.35. (ATCC
CC   staff)
CC   The order of the major features in this plasmid is: bla - HIS3 -
CC   lacZ'/MCS/5'Alu3' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI -
CC   EcoRV - SP6 promoter. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBP109)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH252)
CC   HO (Saccharomyces cerevisiae CGY2516 [ATCC 74013])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   2. human 300bp, Alu repeat
FT                   linker
FT                   -> BLUR8 4700bp
FT                   1. pBP103 BamHI 4600bp, pRS303 2110..2110
FT                   2. BLUR8 BamHI-BamHI 300bp, human Alu repeat
FT                   -> pBP109 4900bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SacII-BstXI-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-
FT                   EcoRI-EcoRV-HindIII-ClaI-SalI-XhoI-ApaI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SacII-EagI-NotI-XbaI-SpeI-
FT                   SmaI-EcoRI-XhoI-ApaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;