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ID   PBP111     preliminary; circular DNA; SYN; 8600 BP.
AC   ATCC77091;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/E.coli plasmid vector pBP111 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   plasmid from YTCA-1x & pSP73
RC   pBP103 from plasmid & pRS303
RC   pBP108, pBP109 from pBP103 & BLUR8
RC   pBP110, pBP111 from pBP103 & pL1.1A
RC   pBP81 from pBP63A & YTCA-1x
RA   Pavan W.J., Hieter P., Sears D., Burkhoff A., Reeves R.H.;
RT   "High-efficiency yeast artificial chromosome fragmentation
RT   vectors";
RL   Gene 106:125-127(1991).
CC   Acentric chromosome fragmentation vector targeting LINE sequences.
CC   Contains promoters for in vitro RNA synthesis. [1]
CC   Constructed from pBP103 (ATCC 77087) by inserting a 4.0 kb
CC   LINE-containing SacI fragment from pL1.1A into the SacI site.
CC   pBP110 (ATCC 77090) and pBP111 (ATCC 77091) differ in the orientation
CC   of the LINE sequence. [1]
CC   Any unique restriction site between the targeting sequence and the
CC   telomere-adjacent sequence can be used to linearize the plasmid before
CC   transformation. [1]
CC   The EcoRV and ClaI sites between the SP6 promoter and the telomere
CC   sequence can be used for recovery from deletion derivatives of YAC
CC   insert sequences adjacent to the fragmentation site. [1]
CC   The order of the major features in this plasmid is: bla - HIS3 -
CC   lacZ'/MCS/3'LINE5' - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI
CC   - EcoRV - SP6 promoter. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   ClaI--4.3 (doublet); EcoRI--7.0, 1.6; SacI--4.6, 4.0;
CC   BamHI--4.7, 2.8, 1.2. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBP111)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH252)
CC   HO (Saccharomyces cerevisiae CGY2516 [ATCC 74013])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 ClaI 4361bp 25..25, ori/amp gene
FT                   2. human ClaI-NotI 4000bp, LINE repeat
FT                   -> pL1.1A 8400bp
FT                   1. pBP103 SacI 4600bp, pRS303 2078..2078
FT                   2. pL1.1A SacI-SacI 4000bp, human LINE repeat
FT                   -> pBP111 8600bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SacII-BstXI-EagI-NotI-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-
FT                   EcoRI-EcoRV-HindIII-ClaI-SalI-XhoI-ApaI-KpnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;