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ID   PBP47      preliminary; circular DNA; SYN; 6400 BP.
AC   ATCC77082;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces/vertebrate/E.coli plasmid vector pBP47 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   HIS3/pSV2neo from pSV2neo & HIS3 gene
RC   BLUR13.RI from BLUR13 & linker
RC   pBP47 from HIS3/pSV2neo & BLUR13.RI
RC   [pPOL2shortneobpA from pBR322 & neo gene]
RC   [pDC47 from pPOL2shortneobpA & pBP47]
RA   Pavan W.J., Hieter P., Reeves R.H.;
RT   "Modification and transfer into an embryonal carcinoma cell line
RT   of a 360-kilobase human-derived yeast artificial chromosome";
RL   Mol. Cell. Biol. 10:4163-4169(1990).
RN   [2]
RC   pL2H3 from pL2 & HIS3 gene
RC   YCTA-1 from yeast telomere
RC   YTCA-1x from YCTA-1 & linker
RC   pC-C1-3A from pC-1/1 & YTCA-1x
RC   pC-C4A2 from pC-1/1 & pTC5
RC   YEpFAT1 from YIp5 & ARS1 & pC-C1-3A & pVZ-1, Drosophila GART gene
RC   YLpFAT1.1 from YEpFAT1 & YTCA-1x
RC   YEpFAT7 from URA3 gene & pC-1/1 & pVZ-1, Drosophila GART gene
RC   YLpFAT7.1 from YEpFAT7 & YTCA-1x
RC   YEpFAT7.4 from YLpFAT7.1
RC   YEpFAT7.5 from YLpFAT7.1
RA   Runge K.W., Zakian V.A.;
RT   "Introduction of extra telomeric DNA sequences into Saccharomyces
RT   cerevisiae results in telomer elongation";
RL   Mol. Cell. Biol. 9:1488-1497(1989).
RN   [3]
RC   BLUR1, BLUR2, BLUR8 from pBR322 & linker & human Alu repeat
RC   BLUR13, BLUR14 from pBR322 & linker & human Alu repeat
RC   pPD9, pPD15, pPD16, pPD17, pPD18 from pBR322 & human repeats
RA   Deininger P.L., Jolly D.J., Rubin C.M., Friedman T., Schmid C.W.;
RT   "Base sequence studies of 300 nucleotide renatured repeated human
RT   DNA clones";
RL   J. Mol. Biol. 151:17-33(1981).
RN   [4]
RC   pL2 from pBR322 & LEU2 gene
RA   Conrad M.;
RT   ;
RL   Unpublished (1989).
RN   [5]
RC   pYt103 from yeast telomere C1-3A sequences
RC   YTCA-1 from pUC9 & pYt103, yeast telomere C1-3A sequences
RA   Gottschling D.;
RT   ;
RL   Unpublished (1989).
RN   [6]
RC   pC-1/1 from yeast 2 micron & leu2-d gene
RA   Irani M., Taylor W.E., Young E.T.;
RT   "Transcription of the ADH2 gene of Saccharomyces cerevisiae is
RT   limited by positive factors that bind competitively to its intact
RT   promoter region on multicopy plasmids";
RL   Mol. Cell. Biol. 7:1233-1241(1987).
RN   [7]
RC   pTC5 from C4A2 sequence
RA   Murray A.W.;
RT   ;
RL   Unpublished (1989).
RN   [8]
RC   pVZ-1 from Drosophila GART gene
RA   Henikoff S., Eghtedarzadeh M.K.;
RT   "Conserved arrangement of nested genes at the Drosophila GART locus";
RL   Genetics 117:711-725(1985).
CC   Constructed by inserting a 1.2 kb 5'-EcoRI/BamHI-3' fragment
CC   containing S. cerevisiae HIS3 into the EcoRI and BamHI sites of
CC   pSV2-neo. [1]
CC   A 0.3 kb BamHI fragment from BLUR13 was modified by adding an EcoRI
CC   site at the SphI site & then cloned into the BamHI site between neoR
CC   and HIS3. [1]
CC   If linearized with EcoRI and transformed into YAC-containing his3
CC   deletion strains, the plasmid will target integration to Alu sequences
CC   and confer a His+ phenotype.  The YAC can then be transferred to a
CC   eukaryotic cell by spheroplast fusion. [1]
CC   YI-type shuttle integrative vector to incorporate a neoR casette into
CC   human-derived (Alu-containing) YACs. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.1, 0.3; EcoRI--6.4; HindIII--3.4, 3.1. (ATCC staff)
CC   The order of the major features in this plasmid is: pMB1 ori - bla -
CC   HIS3 - BamHI - BLUR13.RI - BamHI - neoR. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBP47)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH252)
CC   HO (Saccharomyces cerevisiae CGY2516 [ATCC 74013])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pSV2neo remove EcoRI-BamHI, MCS
FT                   2. yeast -XhoI 1200bp, -296 of HIS3 gene
FT                   EcoRI linker:BamHI linker 1200bp, HIS3 gene
FT                   -> HIS3/pSV2neo
FT                   1. pBR322 BamHI
FT                   2. human, 300bp Alu repeat
FT                   S1 nuclease
FT                   Klenow fill in:Klenow fill in
FT                   BamHI linker:BamHI linker
FT                   -> BLUR13
FT                   1. BLUR13 BamHI-BamHI 300bp, Alu family repeat
FT                   SphI
FT                   EcoRI linker, in Alu family repeat
FT                   -> BLUR13.RI
FT                   1. HIS3/pSV2neo EcoRI
FT                   Klenow fill in
FT                   2. BLUR13.RI BamHI-BamHI 300bp, Alu family repeat
FT                   -> pBP47 6400bp [unique EcoRI]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   repeat          0..0
FT                   /note="REP human Alu family repeat"
FT   CDS             0..0
FT                   /note="ANT Saccharomyces G418 resistance gene (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;