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ID   PBP90C     preliminary; circular DNA; SYN; 9000 BP.
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AC   ATCC77315;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pBP90C - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pBluescript series & HIS3 gene
RC   pBP96 from BLUR8 & plasmid
RC   pBP97 from BLUR8 & plasmid
RC   pBP90 from HIS3 gene & pL1.1A, LINE
RA   Pavan W.J., Reeves R.H.;
RT   "Integrative selection of human chromosome-specific yeast
RT   artificial chromosomes";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:7788-7791(1991).
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RN   [2]
RC   from pBP62
RC   from pYAC3
RC   from pYAC4
RC   from pYAC55
RC   from pJS97
RC   from pJS98
RC   from pYACneo
RC   from pYAC-RC
RC   from pCGS966
RC   from pBP81
RC   from pBP100 series
RC   pBP90 from human LINE repeats
RC   pBP96 from human Alu repeats
RC   pBP97 from human Alu repeats
RC   pBP47 from human Alu repeats & HIS3 & SV40 promoter & neo
RA   Reeves R.H., Pavan W.J., Hieter P.;
RT   "Yeast artificial chromosome modification and manipulation";
RL   Meth. Enzymol. 216:584-603(1992).
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RN   [3]
RC   pL1.1A from LINE repeat
RA   Dombroski B., Mathias S., Nanthakumar E., Scott A., Kazazian H.;
RT   "Isolation of an active human transposable element";
RL   Science 254:1805-1808(1991).
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CC   YI-type shuttle integrative vector targeting human LINE sequences in
CC   YAC libraries derived from somatic cell hybrids. [2]
CC   Constructed by replacing a 2.2 kb XbaI fragment of the LINE element in
CC   pL1.1a with a blunt-ended 1.2 kb fragment containing HIS3. [2]
CC   Linearizing with NotI and ClaI removes plasmid sequences from the LINE
CC   ends for targeting integration. [1]
CC   The NotI site is at the 5' end of the LINE sequence. [1]
CC   The replaced element, nt 3392 to 5607 of the LINE consensus sequence,
CC   contained conserved sequences which might have permitted targeting to
CC   murine-derived YACs. [2]
CC   Restriction digests of the clone give the following sizes (kb):
CC   ClaI--9.3; HindIII--4.9, 2.55, 1.85; XbaI--uncut; NotI--9.3.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBP90C)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (S.cerevisiae YPH252 [ATCC 76621])
CC   HO (S.cerevisiae CYG2516 [ATCC 74013])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript EcoRI 2746bp 424..424, MCS
FT                   Klenow
FT                   2. yeast, -296 of HIS3 gene/pYAC4 5839..5839
FT                   \ pYAC4 1768bp 4238..6006/CDS = complement(4884..5543)
FT                   EcoRI linker 6bp gaattc
FT                   XhoI, end of HIS3 gene/pYAC4 4677..4677
FT                   BamHI linker 6bp ggatcc
FT                   EcoRI-BamHI 1184bp, HIS3 gene/4671..5845
FT                   Klenow:Klenow
FT                   -> plasmid 3950bp
FT                   1. pBR322 ?-ClaI ?..25, ori/amp gene
FT                   2. human ClaI-NotI, LINE repeat
FT                   -> pL1.1A 10000bp
FT                   1. pL1.1A remove XbaI-XbaI 2200bp 3392..5607,
FT                   \ human LINE repeat 7800bp
FT                   blunt end:blunt end
FT                   2. yeast SmaI-EcoRV 1202bp,
FT                   \ pBluescript 424..-..424..442
FT                   blunt end:blunt end
FT                   -> pBP90 9000bp
FT                   1. pBP90
FT                   -> pBP90C 9000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//