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ID   PBPV182    preliminary; circular DNA; SYN; 12000 BP.
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AC   ATCC37110;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pBPV-1(8-2) - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBPV69T from BPV-1
RC   pBPV-1(8-2) from pBR322 & BPV
RA   Howley P.M., Law M.F., Heilman C., Engel L., Alonso M.C.,
RA   Israel M.A., Lowy D.R., Lancaster W.D.;
RT   "Molecular characterization of papillomavirus genomes";
RL   Viruses in Naturally Occurring Cancers 7:233-247(1980).
RL   Essex M., Zur Hausen H., Todaro G., eds.,
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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CC   This vector is suitable for introduction of foreign genes into mouse
CC   mammary tumor cell line C1271 (ATCC CRL 1616). (ATCC staff)
CC   The complete BPV genome was cloned into the BamHI site of pBR322.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBPV-1 8-2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   HO (C127I cell line [ATCC CRL 1616])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. BPV 7600bp, ori
FT                   -> pBPV-1(8-2) 12000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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