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ID   PBPVBETA1  preliminary; circular DNA; SYN; 11500 BP.
AC   IG7007;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pBPV-beta1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBRd from pBR322 & pSVOd
RC   pBPV-H11 from pBPV69T & pBRd
RC   pBPV-beta1, pBPV-beta3 from pBPV-H11 & human beta-globin gene
RA   DiMaio D., Treisman R., Maniatis T.;
RT   "Bovine papilloma virus vector that propagates as a plasmid in
RT   both mouse and  bacterial cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:4030-4034(1982).
CC   Vector for introducing eukaryotic genes into mouse cells and
CC   propagating them as extrachromosomal elements.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBPV-beta1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DH1)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBRd from pBR322)(BPV)(human beta-globin gene)
CC   BR ()
CC   OF (pBVIF)(pBVIFdelta series from pBVIF)(pBPV-H11)(pBPV-deltaR)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. SV40 HindIII-EcoRI 289bp 1494..1783,
FT                   \ ori 0.65 to 0.693
FT                   2. pBR322 remove 1100bp, not ori/3261bp
FT                   HindIII-EcoRI, ori/pBR322 30..-1100bp..4360/3230bp
FT                   -> pSV0d 3519bp
FT                   1. pBR322 BamHI-PstI 3236bp 376..3612
FT                   2. pSVOd remove BamHI-PstI, pBR322 376..3612
FT                   \ [remove 346bp 30..376/748bp 3612..4360]
FT                   -> pBRd 2425bp
FT                   1. pBR322 BamHI-HindIII 4015bp 376..4361..30
FT                   2. BPV HindIII-BamHI 5480bp, 69% fragment
FT                   \ BPV = 7945bp
FT                   -> pBPV69T(43-1) 9500bp
FT                   1. pBPV69T(43-1) HindIII-BamHI 5480bp, BPV 69% frag
FT                   \ BPV = 7945bp
FT                   2. pBRd large HindIII-BamHI 4015bp,
FT                   \ pBR322 376..4361..30
FT                   -> pBPV-H11 9500bp
FT                   1. pBPV-H11 HindIII 9500bp, pBR322 30..30
FT                   2. human HindIII-HindIII 2000bp, beta-globin gene
FT                   -> pBPV-beta1 11500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-PvuI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN human beta-globin gene"
FT   misc_feature    0..0
FT                   /note="BPV 69% fragment"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;