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ID   PBPVBV1    preliminary; circular DNA; SYN; 11500 BP.
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AC   J00218; K00616; M11029; ATCC37225;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pBPV-BV1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBPVbeta10delta1 from pBPVbeta1
RC   pBPV-BV1 from pBPVbeta10delta1 & pBPV-H11
RC   pBVIFdelta series from pBPV-BV1 & human beta-interferon; 12700-13100bp
RA   Zinn K., DiMaio D., Maniatis T.;
RT   "Identification of two distinct regulatory regions adjacent to the
RT   human beta-interferon gene";
RL   Cell 34:865-879(1983).
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CC   Vector for introducing eukaryotic genes into mouse cells and
CC   propagating them as extrachromosomal elements.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBPV-BV1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH1)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBPV-beta1)(pBPV-H11)
CC   BR (pBPV-deltaR)(pBPV-beta1 series)(pBVIF)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBPVbeta1 BamHI, globin gene
FT                   Klenow, fill in
FT                   -> pBPVbeta10delta1
FT                   1. pBPVbeta10delta1 HindIII-HindIII, globin gene
FT                   2. pBPV-H11 BamHI, globin gene
FT                   Klenow, fill in
FT                   HindIII
FT                   calf alkaline phosphatase
FT                   -> pBPV-BV1
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-PvuI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN human beta-globin gene"
FT   misc_feature    0..0
FT                   /note="BPV 69% fragment"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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