back Return to this vector's summary.
ID   PBR313     preliminary; circular DNA; SYN; 5800 BP.
XX
AC   M10197; ATCC37018;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBR313 - incomplete, actinomycin/distamycin.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMB9 from pMB8 & pSC101
RC   pBR312, pBR26 from pMB9 & pSF2124, TnA/Tn3 amp gene
RC   pBR313, pBR315, pBR316 from pBR312
RC   pBR317, pBR318 from pBR313
RA   Bolivar F., Rodriguez R.L., Betlach M.C., Boyer H.W.;
RT   "Construction and characterization of new cloning vehicles, I.
RT   ampicillin-resistant derivatives of the plasmid pMB9";
RL   Gene 2:75-93(1977).
XX
RN   [2]
RP   1-200
RC   from pBR313-SmaF
RA   Wilkins R.J.;
RT   "Selective binding of actinomycin D and distamycin A to DNA";
RL   Nucleic Acids Res. 10:7273-7282(1982).
XX
RN   [3]
RC   pBR313-SmaF from pBR313 & adenovirus II Sma F region
RA   Roeder R.G.;
RT   ;
RL   Unpublished (1982).
XX
CC   A general purpose plasmid vector. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 207771
CC   NM (pBR313)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(E.coli C600)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMB9 5300bp
FT                   insertion of
FT                   2. pSF2124 3000bp, Tn3 amp gene
FT                   -> pBR312 8300bp [amp gene]
FT                   1. pBR312 remove EcoRI*-EcoRI* 300bp, tet promoter
FT                   -> pBR313 5800bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-HpaI-HindIII-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 200 BP; 58 A; 33 C; 43 G; 66 T; 0 other;
     agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
     acgcctattt ttataggtta atgtcatgat aataatggtt tgttagacgt caggtggcac
     ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     gtatccgctc atgagacaat
//