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ID   PBR322GLU  preliminary; circular DNA; SYN; 4362 BP.
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AC   K01492;
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DT   07-NOV-1984 (Rel. 1, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pBR322GLU - incomplete,carcinogen 4362bp EcoRI.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-163
RC   pBR322GLU from pBR322
RA   Hashimoto Y., Shudo K.;
RT   "Sequence selective modification of DNA with muta-carcinogenic
RT   2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole";
RL   Biochem. Biophys. Res. Commun. 116:1100-1106(1983).
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CC   The modification of DNA with O-acylated N-OH-Glu-P-1 is G-specific.
CC   It was deduced that guanines are released in the reaction.
CC   Modification of guanine in DNA with N-OAC-Glu-P-1 occurs more
CC   frequently at the guanine residues in GC cluster-like regions.
CC   Daunomycin (a strong intercalator) inhibits the DNA modification
CC   caused by N-OAc-Glu-P-1.
CC   NCBI gi: 207774
CC   NM (pBR322GLU)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI
FT                   2. gamma-32P-ATP, 5' ends, T4 kinase
FT                   -> pBR322GLU"
FT   rep_origin      0..0
FT                   /note="ORI"
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SQ   Sequence 163 BP; 41 A; 38 C; 35 G; 49 T; 0 other;
     aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
     aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgc
//