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ID   PBR345     preliminary; circular DNA; SYN; 1225 BP.
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AC   K01712; M10848;
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DT   15-DEC-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBR345 - incomplete, replication region.
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KW   cloning vector; replication; origin; origin of replication.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-600
RC   pSC201 from pSC101
RC   pKB410 from pBR333
RC   pKB412 from pSC201 & pBR345
RC   pKB413 from pKB412 & pBR350
RC   pKB418, pKB415, pKB419, pKB417, pKB416 from pKB410 & pSC201
RC   pSY602, pSY403, pSY401, pSY601 from pKB410 & pSC201
RA   Backman K.C., Betlach M.C., Boyer H.W., Yanofsky S.;
RT   "Genetic and Physical Studies on the replication of ColE1-type
RT   plasmids";
RL   Cold Spring Harb. Symp. Quant. Biol. 43:69-76(1979).
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RN   [2]
RP   1-424
RC   pBR345 from pMB1
RA   Bolivar F., Betlach M.C., Heyneker H.L., Shine J., Rodriguez R.L.,
RA   Boyer H.W.;
RT   "Origin of replication of pBR345 plasmid DNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:5265-5269(1977).
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CC   [1] found that the 600 bp segment reported in this entry directs
CC   replication when it is moved to other plasmids. When DNA inserts
CC   were made in this segment, ColE1-type replication was lost. This
CC   sequence differs in only 2 positions from pBR322.
CC   NCBI gi: 207814 & 207815
CC   NM (pBR345)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMB1)(Tn3)
CC   BR ()
CC   OF (pKB412)(pMB20 also from pBR322)(pMB28 from pMB29)
CC   OF (pMB29 from pMB20)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMB1 HaeII-HaeII 363bp, ori
FT                   2. E. coli EcoRI-EcoRI, lac operator
FT                   3. E. coli EcoRI-EcoRI, lac operator
FT                   -> pBR345"
FT   rep_origin      complement(<1..13)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_RNA        454..559
FT                   /note="RNA"
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SQ   Sequence 1084 BP; 229 A; 299 C; 261 G; 235 T; 60 other;
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt tccttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     gaattccact ttaatgcggt agtttatcac agttaaattg ctaacgcagt catcttccgc
     ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagacg gtatcagctc
     actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcag
     agct
//