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ID   PBRG1214   preliminary; circular DNA; SYN; 170 BP.
AC   X05580;
DT   02-APR-1988 (Rel. 3, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pBRG1214 - incomplete, tetracycline leader.
KW   cloning vector; plasmid; tet gene; tetracycline resistance.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-170
RC   pBRG1214 from pBR322 & linker & E. coli lac promoter
RC   pBRG735 from pBR322 & Tn5
RC   pLP103-32-2 from pACYC177 & Mu A gene
RC   pBRG736 from pLP103-32-2 & Tn5
RC   from pBRG1214 & lambda placMu3
RA   Nag D.K., Berg D.E.;
RT   "Specificity of bacteriophage Mu excision";
RL   Mol. Gen. Genet. 207:395-401(1987).
CC   Insert a XhoI linker into a filled-in pBR322 HindIII site
CC   to inactivate the normal tet promoter.
CC   Insert a 96-bp EcoRI fragment containing the lac promoter to restore
CC   tet expression.
CC   See #X05581 and #X05582 for inserted bacteriphage Mu sequences.
CC   NCBI gi: 58121
CC   NM (pBRG1214)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII, tet promoter
FT                   fill in
FT                   XhoI linker
FT                   -> plasmid
FT                   1. plasmid EcoRI
FT                   2. E. coli EcoRI-EcoRI 96bp, lac promoter
FT                   -> pBRG1214"
FT   promoter        0..0
FT                   /note="PRO E. coli lac promoter"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_feature    21..170
FT                   /note="E. coli tet leader"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_feature    complement(25..29)
FT                   /note="SIT bacteriophage lambda plac Mu3 insertion
FT                   site"
FT   misc_feature    93..96
FT                   /note="SIT bacteriophage lambda plac Mu3 insertion
FT                   site"
FT   misc_feature    142..146
FT                   /note="SIT bacteriophage lambda plac Mu3 insertion
FT                   site"
SQ   Sequence 170 BP; 52 A; 33 C; 37 G; 48 T; 0 other;
     ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagaatt
     ctcatgtttg acagcttatc atcgataagc tcctcgagga gctttaatgc ggtagtttat
     cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat