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ID   PBRH3B     preliminary; circular DNA; SYN; 7700 BP.
AC   ATCC37072;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBRH3B - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pBRH1, pBRH3B from pBR316 & oligo
RC   pFTR10 from pBRH1 & pML21
RC   pFTS1 from pBRH1 & pML21
RC   pKTR25 from pML21 & pBR322
RC   pKTS1 from pML21 & pBR322
RC   pBSR series from pBR316 & oligo
RC   pRJR series from pBR316 & oligo
RA   West R.W., Neve R.L., Rodriguez R.L.;
RT   "Construction and characterization of E. coli promoter-probe
RT   plasmid vectors. I. Cloning of promoter-containing DNA fragments";
RL   Gene 7:271-288(1979).
CC   This is a promoter cloning plasmid vector using expression of
CC   tetracycline resistance for selection. (ATCC staff)
CC   This was constructed by inserting an oligonucleotide containing an
CC   EcoRI site into the HindIII site of pBR316, inactivating the tet
CC   promoter. It is resistant to low levels of tetracycline without an
CC   insert and can be used to clone only strong promoters. [2]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBRH3B)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli RR1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pMB9 5300bp
FT                   insertion of
FT                   2. pSF2124 3000bp, Tn3 amp gene
FT                   -> pBR312 8300bp [amp gene]
FT                   1. pBR312 EcoRI*-EcoRI* 300bp, tet promoter 8000bp
FT                   -> pBR316 8000bp [HindIII, no EcoRI]
FT                   1. pBR316 HindIII 8000bp, tet promoter
FT                   S1 nuclease
FT                   EcoRI linker 8bp tgaattca
FT                   -> pBRH3B 7700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 41 BP; 12 A; 6 C; 8 G; 15 T; 0 other;
     tttgacagct tatcatcgat atgaattcat taatgcggta g