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ID   PBRMU1     preliminary; circular DNA; SYN; 73 BP.
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AC   K01211;
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DT   01-OCT-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Mouse/E. coli plasmid vector pBRMU1 - incomplete, L cel HSV TK/pBR322.
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KW   cloning vector; transformant cell line 1-2-75; HSV; thymidine kinase;
KW   L cell; recombinant DNA.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-73
RC   p75X1, p18H11, p17G3, p17G11 from pBR322 & HSV tk gene
RA   Anderson R.A., Kato S., Camerini-Otero R.D.;
RT   "A pattern of partially homologous recombination in mouse L cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:206-210(1984).
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CC   In 3 recombinant junctions, a 17-bp to 19-bp length of filler DNA
CC   was present. There is some homology between the donor sequences near
CC   the junction points in all 6 sequences reported in [1].
CC   NCBI gi: 208966
CC   NM (pBRMU1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mouse L tk+ cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(HSV thymidine kinase)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. HSV, tk gene/#X14112
FT                   2. pBR322
FT                   -> pBRMU1"
FT   misc_recomb     39..40
FT                   /note="hsv tk DNA base 295/filler DNA"
FT   misc_recomb     58..59
FT                   /note="filler DNA/pBR322 DNA base 1380"
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SQ   Sequence 73 BP; 13 A; 28 C; 23 G; 9 T; 0 other;
     atggagccta ggacccagcg agcccggcgc cgccgcggct ccgccgcaag acccaacttg
     ttctgccaag ggt
//