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ID PBRMU2 preliminary; circular DNA; SYN; 73 BP.
DT 01-OCT-1984 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE Mouse/E. coli plasmid vector pBRMU2 - incomplete, L cel HSV TK/HSV TK.
KW cloning vector; transformant cell line 1-2-75; HSV; thymidine kinase;
KW L cell; recombinant DNA.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC p75X1, p18H11, p17G3, p17G11 from pBR322 & HSV tk gene
RA Anderson R.A., Kato S., Camerini-Otero R.D.;
RT "A pattern of partially homologous recombination in mouse L cells";
RL Proc. Natl. Acad. Sci. U.S.A. 81:206-210(1984).
CC In 3 recombinant junctions, a 17-bp to 19-bp length of filler DNA
CC was present. There is some homology between the donor sequences near
CC the junction points in all 6 sequences reported in .
CC NCBI gi: 208967
CC NM (pBRMU2)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)(mouse L tk+ cells)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(HSV thymidine kinase)
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. HSV, tk gene/#X14112
FT 2. pBR322
FT -> pBRMU2"
FT misc_recomb 19..20
FT /note="HSV tk DNA base 2967/HSV tk DNA base 3003"
SQ Sequence 73 BP; 15 A; 23 C; 22 G; 13 T; 0 other;
ggtacgtgta tttctccccg tcgtccagat gcccacgggc cacgtcgagg ccgacgggga