ID   PBRMUA     preliminary; circular DNA; SYN; 52 BP.
XX
AC   K01210;
XX
DT   01-OCT-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mouse/E. coli plasmid vector pBRMUA - incomplete, L cel pBR322/pBR322.
XX
KW   cloning vector; transformant cell line 1-2-75; HSV; thymidine kinase;
KW   L cell; recombinant DNA.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-52
RC   p75X1, p18H11, p17G3, p17G11 from pBR322 & HSV tk gene
RA   Anderson R.A., Kato S., Camerini-Otero R.D.;
RT   "A pattern of partially homologous recombination in mouse L cells";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:206-210(1984).
XX
CC   In 3 recombinant junctions, a 17-bp to 19-bp length of filler DNA
CC   was present. There is some homology between the donor sequences near
CC   the junction points in all 6 sequences reported in [1].
CC   NCBI gi: 208970
CC   NM (pBRMUA)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mouse L tk+ cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(HSV thymidine kinase)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. HSV, tk gene/#X14112
FT                   2. pBR322
FT                   -> pBRMUA"
FT   misc_recomb     20..21
FT                   /note="pBR322 DNA base 1410/filler DNA"
FT   misc_recomb     37..38
FT                   /note="filler DNA/pBR322 DNA base 1204"
XX
SQ   Sequence 52 BP; 13 A; 22 C; 6 G; 11 T; 0 other;
     gtccgccatc tccagcagcc atactcatac tccgcatcta caatccatgc ca
//