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ID   PBRNB      preliminary; circular DNA; SYN; 3276 BP.
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AC   M30906; ATCC37348;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pBRN/B - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBRN/S, pBRN/B from pBR322 & linker
RC   prHu3N/S, prHu5N/S from pBRN/S & human rRNA repeat
RA   Haltiner M.M., Kempe T., Tjian R.;
RT   "A novel strategy for constructing clustered point mutations";
RL   Nucleic Acids Res. 13:1015-1025(1985).
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CC   Plasmid vector for use in generating nested sets of deletion
CC   mutations.
CC   Contains a linker cassette sequence containing NruI and BglII sites
CC   adjacent to each other.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBRN/B)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove 973..2067
FT                   2. BglII-NruI linker cgagatctcgcga
FT                   -> pBRN/B 3276bp [amp]"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII-NruI-PstI-EcoRI-SalI-BamHI-
FT                   ClaI-EcoRV-HindIII-NdeI-
FT                   PvuI-SnaI-SphI-Tth111I-XmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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