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ID PBRS2843 preliminary; circular DNA; SYN; 115 BP.
DT 01-AUG-1985 (Rel. 1, Created)
DT 01-APR-1995 (Rel. 11, Last updated, Version 1)
DE E. coli plasmid vector pBRS2843 - incomplete, T7 A2 promoter/pBR322.
KW cloning vector; drug resistance gene; mutational analysis;
KW promoter region; T7; tetracycline resistance.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pBRS206 from pBRS188 & pOP1, lambda immunity region
RC pBRS242 from pBR322 & pBRS206
RC pBRS240 from pBRS242
RC pBRS246 from pBRS240
RC pBRS2843, pBRS2852 from pBRS240
RC pBRS2845, pBRS2846, pBRS2850 from pBRS240
RC pBRS3077 from pBRS240
RC pBRS2950 from pBRS240
RC pBRS2849 from pBRS240
RC pBRS2343 from pBRS188
RA Savochkina L.P., Retchinsky V.O., Bibilashvili R.S.;
RT "Stability of cloned promoter-containing fragments";
RL Mol. Gen. Genet. 189:142-147(1983).
CC  inserted strong promoters from T7 and lambda into pBR322
CC derived promoter-probe vectors. The inserted promoters, which
CC occurred in dissimilar environments, served as promoters for the
CC pBR322 tetracycline resistance operon. Promoter strength was
CC measured by Tc resistance. Plasmids containing T7 A2 promoters
CC appeared to confer less Tc resistance than those containing A3
CC NCBI gi: 209430
CC NM (pBRS2843)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (T7)(pBR322)
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pBRS240 EcoRI
FT 2. T7 HaeIII-HaeIII 1458bp, prom/leftmost or 4 frag
FT HpaII-HpaII 104, A2 promoter
FT -> pBRS2843"
FT misc_recomb 12..13
FT /note="pBR322 end/T7 start"
FT misc_recomb 95..96
FT /note="T7 end/pBR322 start"
FT promoter 0..0
FT /note="PRO bacteriophage T7 A2 or A3 promoter"
FT promoter 0..0
FT /note="PRO bacteriophage lambda"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
SQ Sequence 115 BP; 44 A; 21 C; 25 G; 25 T; 0 other;
cgtcttcaag aattcggata agtagacagc ctgataagtc gcacgaaaaa caggtattga
caacacgagt aacatgcagt aagatacaaa tcgaattcga taagctttaa tgcgg